• Dewi Masyithah Darlan, Delfitri Munir, Nelva Karmila Jusuf, Agung Putra, Riyadh Ikhsan, and Iffan Alif.
• Department of Parasitology, Faculty of Medicine, Universitas Sumatera Utara (USU), Universitas Sumatera Utara (USU), Medan, Indonesia.
• Med Glas (Zenica). 2020 Aug 1; 17 (2): 408-413.

AbstractAim To analyse the ability of mesenchymal stem cells (MSCs) to regulate interleukin 6 (IL-6) and transforming growth factor (TGF-β) expression in vitro under co-culture conditions in human systemic lupus erythematosus (SLE). Method This study used a post-test group design that used peripheral blood mononuclear cells (PBMCs) from SLE patients at Kariadi Hospital, Semarang, Indonesia, and MSCs from a human umbilical cord. The cells were divided into two groups. The control group of PBMCs was treated with a standard medium, and the treatment group was co-cultured with the MSCs at a 1:40 ratio. Following 24 h incubation, the levels of IL-6 and TGF-β released in the culture medium were measured using a specific ELISA assay. Results This study showed a significant decrease in IL-6 level (p<0.05) and a significant increase in TGF-β level (p<0.001) following 24 h of co-culture incubation of human SLE PBMCs cells and MSCs. Conclusion The PBMCs-to-MSCs ratio of 1:40 can regulate the IL-6 and TGF-β levels in human SLE PBMCs.Copyright© by the Medical Assotiation of Zenica-Doboj Canton.

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