• Fiona P Havers, Carrie Reed, Travis Lim, Joel M Montgomery, John D Klena, Aron J Hall, Alicia M Fry, Deborah L Cannon, Cheng-Feng Chiang, Aridth Gibbons, Inna Krapiunaya, Maria Morales-Betoulle, Katherine Roguski, Mohammad Ata Ur Rasheed, Brandi Freeman, Sandra Lester, Lisa Mills, Darin S Carroll, S Michele Owen, Jeffrey A Johnson, Vera Semenova, Carina Blackmore, Debra Blog, Shua J Chai, Angela Dunn, Julie Hand, Seema Jain, Scott Lindquist, Ruth Lynfield, Scott Pritchard, Theresa Sokol, Lynn Sosa, George Turabelidze, Sharon M Watkins, John Wiesman, Randall W Williams, Stephanie Yendell, Jarad Schiffer, and Natalie J Thornburg.
• CDC COVID-19 Response Team, Centers for Disease Control and Prevention, Atlanta, Georgia.
• JAMA Intern Med. 2020 Jul 21.

ImportanceReported cases of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection likely underestimate the prevalence of infection in affected communities. Large-scale seroprevalence studies provide better estimates of the proportion of the population previously infected.ObjectiveTo estimate prevalence of SARS-CoV-2 antibodies in convenience samples from several geographic sites in the US.Design, Setting, And ParticipantsThis cross-sectional study performed serologic testing on a convenience sample of residual sera obtained from persons of all ages. The serum was collected from March 23 through May 12, 2020, for routine clinical testing by 2 commercial laboratory companies. Sites of collection were San Francisco Bay area, California; Connecticut; south Florida; Louisiana; Minneapolis-St Paul-St Cloud metro area, Minnesota; Missouri; New York City metro area, New York; Philadelphia metro area, Pennsylvania; Utah; and western Washington State.ExposuresInfection with SARS-CoV-2.Main Outcomes And MeasuresThe presence of antibodies to SARS-CoV-2 spike protein was estimated using an enzyme-linked immunosorbent assay, and estimates were standardized to the site populations by age and sex. Estimates were adjusted for test performance characteristics (96.0% sensitivity and 99.3% specificity). The number of infections in each site was estimated by extrapolating seroprevalence to site populations; estimated infections were compared with the number of reported coronavirus disease 2019 (COVID-19) cases as of last specimen collection date.ResultsSerum samples were tested from 16 025 persons, 8853 (55.2%) of whom were women; 1205 (7.5%) were 18 years or younger and 5845 (36.2%) were 65 years or older. Most specimens from each site had no evidence of antibodies to SARS-CoV-2. Adjusted estimates of the proportion of persons seroreactive to the SARS-CoV-2 spike protein antibodies ranged from 1.0% in the San Francisco Bay area (collected April 23-27) to 6.9% of persons in New York City (collected March 23-April 1). The estimated number of infections ranged from 6 to 24 times the number of reported cases; for 7 sites (Connecticut, Florida, Louisiana, Missouri, New York City metro area, Utah, and western Washington State), an estimated greater than 10 times more SARS-CoV-2 infections occurred than the number of reported cases.Conclusions And RelevanceDuring March to early May 2020, most persons in 10 diverse geographic sites in the US had not been infected with SARS-CoV-2 virus. The estimated number of infections, however, was much greater than the number of reported cases in all sites. The findings may reflect the number of persons who had mild or no illness or who did not seek medical care or undergo testing but who still may have contributed to ongoing virus transmission in the population.

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