• Mariana Mendes Porto Azevedo, Alessandra Bernadete Trovó de Marqui, Bruna Tavares Bacalá, Marly Aparecida Spadotto Balarin, Elisabete Aparecida Mantovanni Rodrigues de Resende, Marco Fábio Prata Lima, Mariana Kefalás de Oliveira Gomes, and Mariangela Torreglosa Ruiz Cintra.
• Mestre em Biociências pela Universidade Federal do Triângulo Mineiro/UFTM, Uberaba, MG, Brasil.
• Rev Assoc Med Bras (1992). 2020 Nov 1; 66 (11): 1560-1565.

BackgroundThis study aimed to investigate the deletion polymorphisms of the genes of the glutathione S-transferase family GSTT1 and GSTM1 in patients with Polycystic Ovarian Syndrome (PCOS), comparing them with a control population.MethodsBlood was collected from 219 women (110 with PCOS and 109 controls) and genomic DNA was extracted. For the analysis of polymorphisms, the technique used was multiplex PCR. In the statistical analysis, the chi-square test and multiple logistic regression were used.ResultsThere is no association between the GSTM1 null and GSTT1 null genotypes with PCOS when analyzed separately (P = 0.616 and P = 0.188). The analysis of the combined genotypes showed differences between the groups (P < 0.05), evidencing that the genotypic combination GSTT1 positive and GSTM1 negative is more frequent among patients. In the multivariate analysis, smoking was more frequent in the control group (OR = 0.22; 95% CI - 0.87-0.57; P = 0.002) while the presence of a family history of PCOS (OR = 2, 96; 95% CI - 1.54-5.68; P = 0.001) was more frequent in women with PCOS.ConclusionsIn the studied sample, the deletion polymorphisms of the GSTT1 and GSTM1 genes isolated are not associated with PCOS, but in combination, they may be implicated in the etiology of the condition.

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