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- Alexander Kohlmann, Thomas J Kipps, Laura Z Rassenti, James R Downing, Sheila A Shurtleff, Ken I Mills, Amanda F Gilkes, Wolf-Karsten Hofmann, Giuseppe Basso, Marta Campo Dell'orto, Robin Foà, Sabina Chiaretti, John De Vos, Sonja Rauhut, Peter R Papenhausen, Jesus M Hernández, Eva Lumbreras, Allen E Yeoh, Evelyn S Koay, Rachel Li, Wei-Min Liu, Paul M Williams, Lothar Wieczorek, and Torsten Haferlach.
- Roche Molecular Systems, Inc., Department of Genomics and Oncology, Pleasanton, CA, USA. alexander.kohlmann@roche.com
- Br. J. Haematol. 2008 Sep 1; 142 (5): 802-7.
AbstractGene expression profiling has the potential to enhance current methods for the diagnosis of haematological malignancies. Here, we present data on 204 analyses from an international standardization programme that was conducted in 11 laboratories as a prephase to the Microarray Innovations in LEukemia (MILE) study. Each laboratory prepared two cell line samples, together with three replicate leukaemia patient lysates in two distinct stages: (i) a 5-d course of protocol training, and (ii) independent proficiency testing. Unsupervised, supervised, and r(2) correlation analyses demonstrated that microarray analysis can be performed with remarkably high intra-laboratory reproducibility and with comparable quality and reliability.
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