• Zhongguo Zhong Yao Za Zhi · Aug 2020

    [Comparative study on HPLC fingerprints between crude and processed Ligustri Lucidi Fructus].

    • Hao-Jie Zhang, Huan-Ru Li, Zhi-Yi Feng, Ke Li, Yan-Ping Hu, and Su-Xiang Feng.
    • Henan University of Chinese Medicine Zhengzhou 450046, China Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan & Education Ministry of China Zhengzhou 450046, China Zhengzhou Key Laboratory of Chinese Medicine Quality Control and Evaluation Zhengzhou 450046, China.
    • Zhongguo Zhong Yao Za Zhi. 2020 Aug 1; 45 (16): 3871-3876.

    AbstractTo establish high performance liquid chromatography(HPLC) fingerprints for crude and processed Ligustri Lucidi Fructus,and to evaluate their quality through the similarity calculation and chemical pattern recognition. The separation was performed with Syncronis C_(18) column(4.6 mm × 250 mm, 5 μm), with acetonitrile(A) and 0.1% phosphoric acid solution(B) as the mobile phase for gradient elution, and a detection wavelength of 280 nm. HPLC was used to detect 22 batches of crude and processed Ligustri Lucidi Fructus,and the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 Edition) was used to evaluate the similarity among 22 batches. The research on pattern recognition was conducted with cluster analysis(CA), principal component analysis(PCA), and partial least squares discriminate analysis(PLS-DA). HPLC fingerprints of crude and processed Ligustri Lucidi Fructus were established, with similarity ranging from 0.9 to 1.0. The crude and processed Ligustri Lucidi Fructus can be obviously distinguished by using CA, PCA and PLS-DA. According to the results of PLS-DA,11 constituents including hydroxytyrosol, tyrosol, specnuezhenide and oleuropein were the main marker components leading to the difference. The established fingerprint method is stable and reliable, and can provide method basis for quality control of crude and processed Ligustri Lucidi Fructus. Chemical pattern recognition is proved to be helpful in comprehensive quality control and evaluation of Ligustri Lucidi Fructus before and after the process.

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