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Zhongguo Zhong Yao Za Zhi · Sep 2012
Comparative Study[Research on ginsenosides composition in wild ginseng leaves in different growing years].
- Lan-lan Zhang, Wen-yuan Gao, Zhao-hui Song, Nan Cai, and Shui-ping Zhou.
- College of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, China. zhangll2@tasly.com
- Zhongguo Zhong Yao Za Zhi. 2012 Sep 1; 37 (17): 2530-3.
ObjectiveTo analyze ginsenosides composition in wild ginseng leaves with different growth years.MethodThe analysis was performed on Acquity UPLC BEH Shield RP18 (2.1 mm x 100 mm, 1.7 microm) column, the mobile phase was acetonitrile-0.05% formic acid solution in gradient elution mode. The detection wavelength was at 203 nm. The flow rate was 0.4 mL x min(-1) and column temperature was set at 30 degrees C.ResultThirteen ginsenosides were determined by the established UPLC method. In 5-17th growth year ginseng leaf samples cultivated simulating wild conditions, the contents of ginsenosides in the 14th year have the highest content.ConclusionThe established method is simple, accurate and reliable, can be used in ginsenosides determination and fingerprint research of Panax crude drug. The result provides reliable data for the accumulation of ginsenosides and sustainable utilization of ginseng resources.
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