• Erdem Çokluk, Songül Doğanay, Ramazan ŞekeroğluMehmetMDepartment of Biochemistry, Medical Faculty, Sakarya University, Sakarya, Turkey., Betül TuncerFatımaFDepartment of Biochemistry, Medical Faculty, Sakarya University, Sakarya, Turkey., Hüseyin Çakıroğlu, and Meltem Boz.
• Department of Biochemistry, Medical Faculty, Sakarya University, Sakarya, Turkey.
• Int. J. Clin. Pract. 2021 Nov 1; 75 (11): e14832.

AimsSepsis causes life-threatening tissue and organ dysfunctions caused by endogenous mediators in response to infection. Melatonin is a powerful endogenous anti-inflammatory agent and effective in reducing cellular damage. This study aimed to evaluate the changes in serum and liver tissue levels of VEGF, TGF-β and MMP-2 in melatonin-treated septic rats.Materials And MethodsTwenty-one Wistar-albino male rats were included in this study. Rats were randomly divided into three groups. Group 1 is sham-operated control (C) group, Group 2 is caecal ligation and puncture (CLP) group and Group 3 is melatonin-treated (10 mg/kg) (M-CLP) group. Serum and tissue samples were analysed. All procedures were carried out according to the ethical rules specified in Helsinki Declaration.ResultsSera MMP-2 levels were found higher than tissue MMP-2 levels in C and CLP (respectively, P = .048, P = .01). In CLP and M-CLP, serum TGF-β levels were higher than tissue TGF-β levels(respectively, P = .05, P = .01). Serum VEGF levels in CLP were found to be significantly higher than both C and M-CLP(P < .01).ConclusionMMP-2 levels may have increased because of the prevention of oxidative damage in sepsis, and this may increase the anti-inflammatory effect. Melatonin treatment may have a therapeutic effect against sepsis since it prevents the increase in serum VEGF level. A powerful endogenous antioxidant, may be a promising therapeutic agent on the mortality and morbidity of the disease, because of its lowering effect on serum VEGF, which is a poor prognostic factor in sepsis.© 2021 John Wiley & Sons Ltd.

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