• Sameen Ahmed, Ayesha Vajeeha, Muhammad Idrees, Abrar Hussain, Rakhtasha Munir, Gulshan Zaidi, Khadija Zahid, Rizwan Ahmed, Zareen Fatima, Shazia Rafique, and Niaz M Achakzai.
• Centre of Applied Molecular Biology (CAMB), University of the Punjab, Lahore, Pakistan.
• Medicine (Baltimore). 2023 Jan 13; 102 (2): e32651e32651.

AbstractHigh-risk-human papillomavirus (HR-HPV)-induced cervical cancer is the second most common cause of death among females worldwide. HPV16 is the most prevalent HR-HPV infection worldwide. This study found the genotypic distribution of HR-HPV in the local population and investigated the sequence variations among the E6 and E7 oncogenes of the local HPV16 genotype to the E6 and E7 oncogenes of the foreign HPV16 genotypes and constructed a phylogenetic relationship based on nucleotide sequence comparison among the variants identified in our study along with previously reported isolates that were obtained from different regions of the world. The samples were collected from patients with cervical cancer. Genomic DNA was extracted, and HR-HPV genotypes were determined using real-time PCR. The HPV16 E6 and E7 genes were amplified and sequenced. A HPV16 phylogenetic tree was constructed using the maximum likelihood method with MEGA 7. HPV16 was the most prevalent human papillomavirus (HPV) type identified in the present study. HPV16 isolates belonged to the A1 sublineage of the European branch. Twenty-one nucleotide sequences were included in this analysis. The first, second, and third codon positions are also included. The final dataset included 776 positions.Copyright © 2023 the Author(s). Published by Wolters Kluwer Health, Inc.

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