• Yiming Liu, Mengqiang Song, Hao Yuan, Xianpei Xiao, Jinghao Shu, and Wanlong Pan.
• Faculty of Basic Medicine, North Sichuan Medical University, Nanchong, China.
• Gac Med Mex. 2022 Jan 1; 158 (6): 362368362-368.

IntroductionDendritic cells (DC) are involved in immune recognition, response and immunomodulation mechanisms related to the onset of cancer.ObjectiveTo explore DCs mechanism in the inhibition of autophagy in hepatoma cells.MethodsHuman peripheral blood mononuclear cells were isolated by Ficoll density gradient centrifugation and induced into DCs, which were co-cultured with HepG2 cells by Transwell migration assay. HepG2 cell activity was determined using the CCK8 assay. LC3 autophagy index expression was measured with Western blot analysis, and the expression and secretion of cytokines, with qRT-PCR and ELISA.ResultsIn the co-culture system, DCs were able to reduce HepG2 cells viability; IL-2, IL-12, IL-10 and IFN-γ expression in DCs was also significantly inhibited, although IL-2 and IFN-γ were still expressed 0.6 and 0.53 more than in the control group.ConclusionDCs can regulate autophagy in hepatocellular carcinoma cells. The mechanism may be related to the synthesis and release of cytokines such as IL-2, IL-12 and IFN-γ by DCs.Copyright: © 2022 Permanyer.

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