• Jeremy Epah, Gabriele Spohn, Kathrin Preiß, Markus M Müller, Johanna Dörr, Rainer Bauer, Shabnam Daqiq-Mirdad, Joachim Schwäble, Stefanie N Bernas, Alexander H Schmidt, Erhard Seifried, and Richard Schäfer.
• Institute for Transfusion Medicine and Immunohaematology, German Red Cross Blood Donor Service Baden-Württemberg-Hessen gGmbH, Goethe University Hospital, Frankfurt Am Main, Germany.
• Bmc Med. 2023 Sep 19; 21 (1): 360360.

BackgroundBone marrow (BM) transplantation is a life-saving therapy for hematological diseases, and the BM harbors also highly useful (progenitor) cell types for novel cell therapies manufacture. Yet, the BM collection technique is not standardized.MethodsBenchmarking our collection efficiency to BM collections worldwide (N = 1248), we noted a great variability of total nucleated cell (TNC) yields in BM products (HPC-M) with superior performance of our center, where we have implemented a small volume aspirate policy. Thus, we next prospectively aimed to assess the impact of BM collection technique on HPC-M quality. For each BM collection (N = 20 donors), small volume (3 mL) and large volume (10 mL) BM aspirates were sampled at 3 time points and analyzed for cell composition.ResultsCompared to large volume aspirates, small volume aspirates concentrated more TNCs, immune cells, platelets, hematopoietic stem/progenitor cells, mesenchymal stromal cells (MSCs), and endothelial progenitors. Inversely, the hemoglobin concentration was higher in large volume aspirates indicating more hemoglobin loss. Manufacturing and dosing scenarios showed that small volume aspirates save up to 42% BM volume and 44% hemoglobin for HPC-M donors. Moreover, MSC production efficiency can be increased by more than 150%.ConclusionsWe propose to consider small volume BM aspiration as standard technique for BM collection.© 2023. BioMed Central Ltd., part of Springer Nature.

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