• Rong Huang, Rongfeng Xu, Rui Zhang, Wenjie Zuo, Zhenjun Ji, Zaixiao Tao, Yongjun Li, and Genshan Ma.
• Department of Cardiology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, China; Department of Cardiology, Affiliated Hospital of Nantong University, Nantong, China. Electronic address: hrseu1988@163.com.
• Clinics (Sao Paulo). 2024 Jan 1; 79: 100410100410.

BackgroundCuproptosis is known to regulate diverse physiological functions in many diseases, but its role in regulating Myocardial Ischemia-Reperfusion Injury (MI/RI) remains unclear.MethodsFor this purpose, the MI/RI microarray datasets GSE61592 were downloaded from the Gene Expression Omnibus (GEO) database, and the Differently Expressed Genes (DEGs) in MI/RI were identified using R software. Moreover, the MI/RI mice model was established to confirm further the diagnostic value of Pyruvate Dehydrogenase B (Pdhb), Dihydrolipoamide S-acetyltransferase (Dlat), and Pyruvate dehydrogenase E1 subunit alpha 1 (Pdhα1).ResultsThe analysis of microarray datasets GSE61592 revealed that 798 genes were upregulated and 768 were downregulated in the myocardial tissue of the ischemia-reperfusion injury mice. Furthermore, Dlat, Pdhb, Pdhα1, and cuproptosis-related genes belonged to the downregulated genes. The receiver operating characteristics curve analysis results indicated that the Dlat, Pdhb, and Pdhα1 levels were downregulated in MI/RI and were found to be potential biomarkers for MI/RI diagnosis and prognosis. Similarly, analysis of Dlat, Pdhb, and Pdhα1 levels in the MI/RI mice revealed Pdhb being the key diagnostic marker.ConclusionsThis study demonstrated the prognostic value of cuproptosis-related genes (Dlat, Pdhb, and Pdhα1), especially Pdhb, MI/RI, providing new insight into the MI/RI treatment.Copyright © 2024 HCFMUSP. Published by Elsevier España, S.L.U. All rights reserved.

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