• Clinics · Jan 2024

    Improvement of motor function in mice after implantation of mononuclear stem cells from human umbilical cord and placenta blood after 3 and 6 weeks of experimental spinal cord injury.

    • AraujoThiego Pedro FreitasTPFDepartment of Orthopedic Surgery, Hospital Sírio Libanês, Brasília, DF, Brazil. Electronic address: thiegopedro@gmail.com., Alexandre Fogaça Cristante, Raphael Martus Marcon, SantosGustavo Bispo DosGBDDepartment of Orthopedic Surgery, Instituto de Ortopedia e Traumatologia da Universidade de São Paulo, São Paulo, SP, Brazil., Maria Helena Alves Nicola, Alex Oliveira de Araujo, Fernando Barbosa Sanchez, and Barros FilhoTarcísio Eloy Pessoa deTEPDepartment of Orthopedic Surgery, Instituto de Ortopedia e Traumatologia da Universidade de São Paulo, São Paulo, SP, Brazil..
    • Department of Orthopedic Surgery, Hospital Sírio Libanês, Brasília, DF, Brazil. Electronic address: thiegopedro@gmail.com.
    • Clinics (Sao Paulo). 2024 Jan 1; 79: 100509100509.

    Study DesignExperimental study utilizing with a standardized model (MASCIS Impactor) of Spinal Cord Injury (SCI) in Balb C mouse model with implantation of mononuclear stem cells derived from the human umbilical cord and placenta blood in the early chronic phase of SCI.ObjectivesThe aim of this study was to evaluate the nerve regeneration and motor functional recovery in Balb C mice with surgically induced paraplegia in response to the use of mononuclear stem cells, in early chronic phase (> 2 weeks and < 6 months), because there is yet potential of neuronal and functional recovery as the neuronal scar is not still completely established.MethodsForty-eight mice were randomly assigned to 6 groups of 8 animals. Group 1 received the stem cells 3 weeks after the trauma, and Group 2 received them six weeks later. In Group 3, saline solution was injected at the site of the lesion 3 weeks after the trauma, and in Group 4, 6 weeks later. Group 5 underwent only spinal cord injury and Group 6 underwent laminectomy only. The scales used for motor assessment were BMS and MFS for 12 weeks.ResultsThe intervention groups showed statistically significant motor improvement. In the histopathological analysis, the intervention groups had a lower degree of injury (p < 0.05). Regarding axonal budding, the intervention groups showed increasing in axonal budding in the caudal portion (p < 0.05).ConclusionsThe use of stem cells in mice in the chronic phase after 3 and 6 weeks of SCI brings functional and histopathological benefits to them.Copyright © 2024 HCFMUSP. Published by Elsevier España, S.L.U. All rights reserved.

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