• Clinics · Jan 2024

    Observational Study

    Protocol for the collection, packaging, and transportation of preterm newborn stool samples.

    • Jessica Santos Passos Costa, Heli Vieira Brandão, Mara Viana Cardoso Amaral, Gabriela Cintra Dos Santos, Camilla da Cruz Martins, Michelle de Santana Xavier Ramos, Tatiana de Oliveira Vieira, Raquel Guimarães Benevides, and Graciete Oliveira Vieira.
    • Health Research and Extension Center, Postgraduate Program in Public Health, Department of Health, Universidade Estadual de Feira de Santana, Feira de Santana, BA, Brazil; PhD in Public Health, Universidade Estadual de Feira de Santana, Feira de Santana, BA, Brazil. Electronic address: jessy17_sp@hotmail.com.
    • Clinics (Sao Paulo). 2024 Jan 1; 79: 100531100531.

    BackgroundThe newborn's intestinal microbiota can vary with gestational age. Reliable analyses of stool samples require establishing the steps of collection, packaging, transportation, and storage.ObjectiveTo describe the development of a protocol and test an algorithm for the sequence of actions and procedures for the collection, packaging, transportation, and storage of stool samples from Preterm Newborn (PTNB) admitted to a Neonatal Intensive Care Unit (NICU).Materials And MethodsThis is a descriptive, observational study linked to a non-randomized controlled clinical trial conducted in the NICU of two public hospitals covenanted to the Unified Health System of a large city in inland northeastern Brazil. The methodology adopted in its development followed the Brazilian Ministry of Health Preparation Guide recommendations.ResultsThe flow of actions and procedures was consistent with scientific evidence. The sequence of work process steps to implement the proposed protocol was tested and resulted in a graphical representation of an algorithm compatible with the reality of Brazilian public hospitals.ConclusionFollowing the protocol steps with the description of the rules of conduct and recommendations regarding the collection, packaging, transportation, and storage of stool samples from PTNBs ensured the preservation and integrity of the bacterial DNA in the stools sample. The details of the recommendations will allow their reproducibility and improvement by professionals and researchers with similar study objects.Trial RegistrationWorld Health Organization (WHO) under Universal Trial Number (UTN) code U1111-1266-2295, under register RBR-3mm7cs in the Brazilian Registry of Clinical Trials (REBEC).Copyright © 2024. Published by Elsevier España, S.L.U.

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