• Bari R Morris, Armelle deLaforcade, Joyce Lee, Joseph Palmisano, Dawn Meola, and Elizabeth Rozanski.
• Department of Clinical Sciences, Tufts Cummings School of Veterinary Medicine, North Grafton, MA, 01536.
• J Vet Emerg Crit Care (San Antonio). 2016 Jan 1; 26 (1): 58-63.

ObjectiveTo investigate the effects of in vitro hemodilution with lactated Ringers solution (LRS), hetastarch (HES), and fresh frozen plasma (FFP) on whole blood coagulation in dogs as assessed by kaolin-activated thromboelastography.DesignIn vitro experimental study.SettingUniversity teaching hospital.AnimalsSix healthy client-owned dogs.InterventionsWhole blood was collected and diluted in vitro at a 33% and 67% dilution with either LRS, HES, or FFP.Measurements And Main ResultsKaolin-activated thromboelastography was performed on each sample as well as a control. Thromboelastographic parameters R (min), alpha (deg), K (min), and MA (mm) were measured and compared to the sample control for each dilution using mixed model methodology. Prolongation in coagulation times were seen at both dilutions with LRS and HES. There was no significant difference in R times at the 33% dilution, but R time was significantly prolonged at the 67% dilution with HES (P = 0.004). MA was significantly decreased for LRS at both dilutions (P = 0.013, P < 0.001) and more profoundly decreased for HES (P < 0.001, P = 0.006). No significant difference in any parameter was found for FFP.ConclusionsIn vitro hemodilution of whole blood with both LRS and HES but not FFP resulted in significant effects on coagulation with HES having a more profound effect. In vivo evaluation of changes in coagulation with various resuscitation fluids is warranted and may be clinically relevant.© Veterinary Emergency and Critical Care Society 2015.

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