• Meirong Yang, Haitao Wang, Guangwei Han, Lianhua Chen, Lina Huang, Jihong Jiang, and Shitong Li.
• Department of Anesthesiology, First People's Hospital, School of Medicine, Shanghai Jiaotong University, 100 Hai Ning Road, Shanghai, 200080, China.
• Muscle Nerve. 2013 Dec 1; 48 (6): 958-62.

IntroductionWe investigated a novel application of phrenic nerve stimulation (PNS) in diaphragm dysfunction induced by mechanical ventilation (MV).MethodsTwenty-one Sprague-Dawley rats were assigned randomly to 3 groups: spontaneous breathing, 18-h controlled MV, and 18-h controlled MV with PNS. Upon completion of the experimental protocol, diaphragm contractility, gene expression of insulin-like growth factor-1 (IGF-1) and ubiquitin ligases, and serum IGF-1 levels were analyzed.ResultsCompared with the spontaneously breathing rats, impaired diaphragm contractile function, including force-related properties and force-frequency responses, were pronounced with MV. Furthermore, MV suppressed IGF-1 and induced muscle ring finger 1 mRNA expression in the diaphragm. In contrast, PNS counteracted MV-induced gene expression changes in the diaphragm and restored diaphragm function.ConclusionsPNS exerted a protective effect against MV-induced diaphragm dysfunction by counteracting altered expression of IGF-1 and ubiquitin ligase in the diaphragm.Copyright © 2013 Wiley Periodicals, Inc.

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