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J. Clin. Microbiol. · Dec 1997
Rapid discrimination of Mycobacterium tuberculosis complex strains by ligation-mediated PCR fingerprint analysis.
- G Prod'hom, C Guilhot, M C Gutierrez, A Varnerot, B Gicquel, and V Vincent.
- Centre National de Référence des Mycobactéries, Institut Pasteur, Paris, France.
- J. Clin. Microbiol. 1997 Dec 1; 35 (12): 3331-4.
AbstractA ligation-mediated PCR (LMPCR) method for the amplification of sequences flanking the IS6110 of the Mycobacterium tuberculosis complex has been developed. The method uses one primer specific for IS6110 and a second specific for a linker ligated to SalI-restricted genomic DNA. LMPCR is a rapid screening method, valuable for the fingerprinting of M. tuberculosis complex strains.
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