• Ahmet Özer Şehirli, Burcu Satılmış, Şermin Tetik, Şule Çetinel, Berrak Yeğen, Aslı Aykaç, and Göksel Şener.
• Department of Pharmacology, Marmara University Faculty of Pharmacy, İstanbul-Turkey; Near East University, Faculty of Dentistry, Lefkoşa-KKTC. ozersehirli@hotmail.com.
• Ulus Travma Acil Cer. 2016 Sep 1; 22 (5): 417-422.

BackgroundThis study was designed to determine possible protective effect of betaine treatment against oxidative injury in pulmonary tissue induced with thermal trauma.MethodsUnder ether anesthesia, shaved dorsum of Wistar albino rats was exposed to a 90°C water bath for 10 seconds to induce burn injury. Betaine was administered orally (250 mg/kg) for a period of 21 days before burn injury, and single dose of betaine was administered after thermal injury. Control group rats were exposed to 25°C water bath for 10 seconds. Upon conclusion of experiment, rats were decapitated and blood was collected for analysis of pro-inflammatory cytokines and lactate dehydrogenase (LDH) activity. Lung tissue samples were taken to determine malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO), and Na+/K+-ATPase activity, in addition to histological analysis.ResultsBurn injury caused significant increase in both cytokine levels and LDH activity. In lung samples, raised MDA levels, MPO activity, and reduced GSH levels and Na+/K+-ATPase activity were found due to burn injury.ConclusionTreatment of rats with betaine significantly restored GSH level and Na+/K+-ATPase activity, and decreased MDA level and MPO activity. According to the findings of the present study, betaine significantly diminishes burn-induced damage in tissue.

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