• Matteo Goldoni, Andrea Caglieri, Roberta Andreoli, Diana Poli, Paola Manini, Maria Vittoria Vettori, Massimo Corradi, and Antonio Mutti.
• National Institute of Occupational Safety and Prevention, Research Center at the University of Parma, Parma, Italy. matgold@libero.it
• BMC Pulm Med. 2005 Sep 1; 5: 10.

BackgroundThe effects of changes in cooling temperature on biomarker levels in exhaled breath condensate have been little investigated. The aim of the study was to test the effect of condensation temperature on the parameters of exhaled breath condensate and the levels of selected biomarkers.MethodsExhaled breath condensate was collected from 24 healthy subjects at temperatures of -10, -5, 0 and +5 C degrees. Selected parameters (condensed volume and conductivity) and biomarkers (hydrogen peroxide, malondialdehyde) were measured.ResultsThere was a progressive increase in hydrogen peroxide and malondialdehyde concentrations, and condensate conductivity as the cooling temperature increased; total condensate volume increased as the cooling temperature decreased.ConclusionThe cooling temperature of exhaled breath condensate collection influenced selected biomarkers and potential normalizing factors (particularly conductivity) in different ways ex vivo. The temperature of exhaled breath condensate collection should be controlled and reported.

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