Croatian medical journal
-
Croatian medical journal · Jun 2009
Genetic polymorphism and haplotype analysis of 4 tightly linked X-STR duos in Koreans.
To investigate genetic polymorphism and haplotypes of tightly linked X-chromosomal short tandem repeat (X-STR) clusters in Koreans. ⋯ Four X-STR duos in the linkage group 1-4 will be able to provide a powerful tool for solving complex kinship cases in Koreans. However, to increase the haplotype diversity in the linkage group 4, it will be useful to discover a new marker for Asians that can serve as an adequate substitute for DXS7423 or at least complement the existing linkage duo of DXS10134-DXS7423.
-
Croatian medical journal · Jun 2009
Genetic polymorphisms of 17 Y-chromosomal short tandem repeat loci in Atayal population of Taiwan.
To define the Y-chromosomal genetic structure in a sample of Atayal men from Taiwan. ⋯ Our results will help provide the molecular genetic evidence for human settlement of the Pacific.
-
Croatian medical journal · Jun 2009
Population data for 15 short tandem repeat loci from Wallachia Region, South Romania.
To determine allele frequencies' distribution for 15 short tandem repeat (STR) loci in a population sample of 1910 unrelated individuals from the region of Wallachia, South Romania. ⋯ According to 15 analyzed STR loci, the population of Wallachia region was found to be genetically more similar to Slavic populations of Croatia and Serbia than to other surrounding populations.
-
Croatian medical journal · Jun 2009
Developmental validation of short tandem repeat reagent kit for forensic DNA profiling of canine biological material.
To develop a reagent kit that enables multiplex polymerase chain reaction (PCR) amplification of 18 short tandem repeats (STR) and the canine sex-determining Zinc Finger marker. ⋯ The kit, which produces robust, reliable, and reproducible results, will be made available for the forensic research community after modifications based on this study's evaluation to comply with the quality standards expected for forensic casework.
-
Croatian medical journal · Jun 2009
ReviewValidity of low copy number typing and applications to forensic science.
Low copy number (LCN) typing, particularly for current short tandem repeat (STR) typing, refers to the analysis of any sample that contains less than 200 pg of template DNA. Generally, LCN typing simply can be defined as the analysis of any DNA sample where the results are below the stochastic threshold for reliable interpretation. There are a number of methodologies to increase sensitivity of detection to enable LCN typing. ⋯ While LCN typing is appropriate for identification of missing persons and human remains and for developing investigative leads, caution should be taken with its use in other endeavors until developments are made that overcome the vagaries of LCN typing. A more in-depth evaluation by the greater scientific community is warranted. The issues to consider include: training and education, evidence handling and collection procedures, the application or purpose for which the LCN result will be used, the reliability of current LCN methods, replicate analyses, interpretation and uncertainty, report writing, validation requirements, and alternate methodologies for better performance.