American journal of veterinary research
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Comparative Study
Comparison of techniques for determination of chondrocyte viability after thermal injury.
To compare 2 methods of quantitating chondrocyte viability and to determine chondrocyte response to thermal injury over time. ⋯ CLM and LDH histochemistry techniques yield different absolute numbers of live and dead cells, resulting in differing percentages of live or dead cells with each technique. These differences may be related to the enzymes responsible for activation in each technique and the susceptibility of these enzymes to thermal injury. Results of TUNEL indicate that apoptosis contributes to chondrocyte death after thermal injury, with a peak signal identified 3 days after insult.