Brain research. Brain research protocols
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Brain Res. Brain Res. Protoc. · Apr 2000
Methods to induce primary and secondary traumatic damage in organotypic hippocampal slice cultures.
Organotypic brain slice cultures have been used in a variety of studies on neurodegenerative processes [K. M. Abdel-Hamid, M. ⋯ Asseline, Effect of mild hypothermia on cerebral energy metabolism during the evolution of hypoxic ischaemic brain damage in the immature rat, Stroke, 5, 1996, pp. 919-925.]. Low temperature incubation significantly reduced cell death, now being 9% at 24 h and 14% at 67 h. Our results show that these models of moderate mechanical trauma using organotypic slice cultures can be used to study neurodegeneration and neuroprotective strategies.