Lancet
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The first successful attempt at deep freezing and thawing of the human oocyte is reported. A twin pregnancy was achieved after insemination and replacement in utero. ⋯ Thawing was achieved rapidly by warming in a 37 degrees C water-bath, followed by dilution of dimethyl sulphoxide as a single step. 80% of forty oocytes showed morphological survival after freezing and thawing. Thirty of these were inseminated; 83% retained their capacity to be fertilised, and 60% proceeded to cleavage division.
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Tympanometry was used to identify middle-ear changes in 154 children with otitis media during and after treatment in general practice. In two hundred and nineteen episodes, 242 infected and 196 non-infected ears were examined. The results suggest that chronic serous otitis media or "glue ear" is not a consequence of acute otitis media; that persistence of middle-ear effusion is unlikely to predispose to recurrent acute otitis media; and that in unilateral otitis media abnormalities are often present in both ears.