The International journal of neuroscience
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Morphological changes in the dopamine neurons and glial cells of the rat mid-brain ascending dopamine pathways were investigated after a partial lesion induced by unilateral striatal injection of a small dose of 6-hydroxydopamine (6-OHDA). Fourteen days after lesion, animals showed contralateral rotation induced by apomorphine injection. After behavioral analysis, fats were killed and their brains processed for the immunohistochemistry tyrosine hydroxylase (TH), a marker for dopamine cells, as well as glial fibrillary acidic protein (GFAP) and OX-42, markers for astrocyte and microglia, respectively. ⋯ The rotator detected cytoplasmic hypertrophy in the astrocytes, and also a shift to the fight of the gaussian curves of the normal distribution of the logarithmic plotted values of the astroglial cell body volumes of the neostriatum bilaterally as well as in the ipsilateral SNc and VTA of the striatal 6-OHDA injected rats. Cytoplasmic hypertrophy of microglia, and also a shift to the right of the gaussian curves of the values of microglia cell body volumes were seen only in the ipsilateral neostriatum; however, the point intercepts revealed an increased amount of microglial processes in the ipsilateral SNc and VTA of the lesioned rats. Specific stereological methods can be applied on detection of regionally different forms of cellular astroglial and microglial reaction after a partial lesion of dopamine pathway.
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Comparative Study
Effects of tramadol on nerve action potentials in rat: comparisons with benzocaine and lidocaine.
The effects of tramadol on repetitively elicited action potentials were studied in rat sciatic nerve, using the sucrose gap method. Tramadol's local anesthetic-like effects were compared with lidocaine and benzocaine at single or 10, 40, and 100 Hz stimulations. Tramadol and lidocaine both produced approximately the same level of conduction block. ⋯ However, their frequency-dependent block patterns were similar. It was concluded that tramadol may block the Na+ channels following the hydrophilic pathway like lidocaine and block K+ channels more than lidocaine. These may accounted for the local anesthetic-like effects of tramadol.
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Comparative Study
Chronic treatment with clomipramine and desipramine induces deficit in long-term visuo-spatial memory of rats.
Although the effects of antidepressants in brain neurochemistry have been extensively studied, there are scarce and inconsistent data on the effect of these drugs in learning and memory. The authors studied the effect of daily administration of a single dose of either clomipramine or desipramine, two monoamine-reuptake-inhibitors with preferential serotonergic and noradrenergic profiles, respectively, during 15 days, on the visuo-spatial memory of adults rats measured through their performance in an eight-arm radial maze. Rats receiving 10 mg/kg i.p. daily of clomipramine or desipramine, 30 min before testing, committed a significantly greater number of errors than saline-treated control rats throughout the duration of the test (5 sessions, 15 days), excepting for session one (after 3 days of testing) where there were no differences between the 3 groups of rats. Results indicated that both serotonergic and noradrenergic antidepressants could impair long-term visuo-spatial memory in the rat, whereas inducing no changes in working memory, effects that are likely related to changes in brain monoamine metabolism induced by the antidepressant drugs.
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Review Comparative Study
Spinal cord injury: reversing the incorrect cortical maps by inductive lability procedure.
Within the brain-stem and on the cerebral cortex there are locomotor control centers arranged in a ladder-form control system. These centers are somatotopic, self-organizing neural network maps capable of simultaneously learning and task execution. ⋯ The treatment method based on inductive lability procedure (Krishnan, 2003a, 2003b, 2003c) uses botulinum toxin for the purpose. It recreates competition among synapses in a locomotor training-based corrective re-self-organization of the maps in various steps of the ladder.
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Comparative Study
Effect of long-term ketamine administration on vocalization to paw pressure and on spinal wind-up activity in monoarthritic rats.
The antinociceptive effect of long-lasting ketamine administration (mini-osmotic pump) was studied in monoarthritic rats by using hindpaw pressure testing and wind-up measurement in a C-fiber reflex paradigm. Chronic ketamine induced antinociception in the monoarthritic paw and significantly suppressed mechanical hyperalgesia during the 14-day treatment period. ⋯ After pump removal, vocalization thresholds and spinal wind-up scores from the monoarthritic paw returned to control values, while hyperalgesia developed in the normal paw. Results suggest that ketamine upregulates NMDA receptors upon long-term administration, resulting in hyperalgesic response in the normal paw after drug withdrawal.