Journal of neuroscience methods
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J. Neurosci. Methods · Sep 2011
Comparative StudyDevelopment of mechanical and thermal nociceptive threshold testing devices in unrestrained birds (broiler chickens).
Behavioural signs of pain are difficult to quantify and interpret in animals. Nociceptive threshold testing is therefore a useful method for examining the perception and processing of noxious stimuli underlying pain states. Devices were developed to measure response thresholds to quantified, ramped mechanical and thermal nociceptive stimuli applied to the leg or keel of unrestrained birds. ⋯ Individual birds' mechanical keel thresholds were also repeatable across sessions. The apparatus gave reliable, reproducible measurements of thresholds to noxious mechanical and thermal stimuli. The range recorded was comparable with previously published nociceptor thresholds in dissected chicken nerve filament fibres, and the method appears suitable for studying nociceptive processes in broiler chickens.
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J. Neurosci. Methods · Sep 2011
Comparative StudyOrganotypic vibrosections: novel whole sagittal brain cultures.
In vitro cell culture models are of enormous importance in neuroscience research and organotypic brain slices are found to be a potent model very close to the in vivo situation. Brain slices can be cultured as single slices or as co-slices. However, there is need to culture whole brain sections, containing the complex functional architecture. ⋯ In conclusion, our novel model provides an easy potent whole sagittal brain culture system that allows to study cholinergic and dopaminergic neurons together but also in close interaction with all other cells of the brain and with capillaries. It will be a great challenge in future to use this model to re-construct whole pathways. This vibrosection model may partly represent a close adult in vivo situation, which allows to study neurodegeneration and neuroprotection of cholinergic and dopaminergic neurons, which plays an important role in Alzheimer's and Parkinson's disease, respectively.
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J. Neurosci. Methods · Sep 2011
Automated Facial Action Coding System for dynamic analysis of facial expressions in neuropsychiatric disorders.
Facial expression is widely used to evaluate emotional impairment in neuropsychiatric disorders. Ekman and Friesen's Facial Action Coding System (FACS) encodes movements of individual facial muscles from distinct momentary changes in facial appearance. Unlike facial expression ratings based on categorization of expressions into prototypical emotions (happiness, sadness, anger, fear, disgust, etc.), FACS can encode ambiguous and subtle expressions, and therefore is potentially more suitable for analyzing the small differences in facial affect. ⋯ Applicability of the automated FACS was illustrated in a pilot study, by applying it to data of videos from eight schizophrenia patients and controls. We created temporal AU profiles that provided rich information on the dynamics of facial muscle movements for each subject. The quantitative measures of flatness and inappropriateness showed clear differences between patients and the controls, highlighting their potential in automatic and objective quantification of symptom severity.
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J. Neurosci. Methods · Aug 2011
A surgical device for minimally invasive implantation of experimental deep brain stimulation leads in large research animals.
Deep brain stimulation (DBS) in experimental animals has promoted new indications and refined existing treatments. Implantation of downscaled clinical DBS leads directly compatible with commercially available implantable pulse generators can however be challenging. Accordingly, we have developed a lead implantation device (LID) and technique for minimally invasive implantation of experimental multicontact DBS leads in large research animals. ⋯ The LID was easy to handle and capable of accurate stereotaxic implantation of downscaled experimental DBS leads in the predetermined target brain structures with minimal surrounding tissue reaction. The device may benefit future large animal DBS research as it allows for precise implantation of DBS leads and may have implications for further refinement of clinical DBS leads.
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J. Neurosci. Methods · Jul 2011
Direct injection into the dorsal root ganglion: technical, behavioral, and histological observations.
Direct injection of agents into the dorsal root ganglia (DRGs) offers the opportunity to manipulate sensory neuron function at a segmental level to explore pathophysiology of painful conditions. However, there is no described method that has been validated in detail for such injections in adult rats. We have found that 2 μl of dye injected through a pulled glass pipette directly into the distal DRG, exposed by a minimal foraminotomy, produces complete filling of the DRG with limited extension into the spinal roots. ⋯ Injection of adeno-associated virus (AAV) vector conveying green fluorescent protein (GFP) transgene resulted in expression as soon as 1 day after injection into the DRG, including fibers in the spinal dorsal horn and columns. AAV injection into the DRG produced additional thermal hypersensitivity and withdrawal from the stroke of a brush and compromised motor performance. These findings demonstrate a method for selective injection of agents into single DRGs for anatomically restricted actions.