Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv für klinische und experimentelle Ophthalmologie
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Graefes Arch. Clin. Exp. Ophthalmol. · Dec 2011
Real-time polymerase chain reaction and intraocular antibody production for the diagnosis of viral versus toxoplasmic infectious posterior uveitis.
The aim of this work was to determine the diagnostic performance of real-time polymerase chain reaction (RT-PCR) and to assess intraocular specific antibody secretion (Goldmann-Witmer coefficient) on samples from patients with signs of posterior uveitis presumably of infectious origin and to target the use of these two biologic tests in the diagnostic of Toxoplasma/viral Herpesviridae posterior uveitis by the consideration of clinical behavior and delay of intraocular sampling. ⋯ For the diagnosis of viral or toxoplasmosis-associated intraocular inflammation, the usefulness of laboratory diagnosis tools (RT-PCR and GWC) depends on parameters other than the sensitivity of the tests. Certain patient characteristics such as the age of the patients, immune status, duration since the onset of symptoms, retinitis area, predominant site and extent of inflammation within the eye should orientate the rational for the choice of laboratory testing in analysis of intraocular fluids.
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Graefes Arch. Clin. Exp. Ophthalmol. · Nov 2011
Comparative StudyConfocal laser-scanning microscopy allows differentiation between Fabry disease and amiodarone-induced keratopathy.
The aim of this work is to compare the microstructure of cornea verticillata in Fabry disease with amiodarone-induced keratopathy by means of in vivo confocal laser-scanning microscopy (CLSM). ⋯ While Fabry-induced cornea verticillata and amiodarone keratopathy cannot be distinguished by conventional slit-lamp microscopy, CLSM allows the differentiation between both etiologies in the majority of patients. CLSM appears to reveal corneal changes prior to the detection of cornea verticillata on slit-lamp microscopy and may thus be helpful in the early diagnosis of Fabry disease. CLSM does not allow quantitative monitoring of corneal changes in Fabry patients under enzyme-replacement therapy.
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Graefes Arch. Clin. Exp. Ophthalmol. · Oct 2011
Randomized Controlled Trial Multicenter Study Comparative StudyPain during second eye cataract surgery under topical anesthesia: an intraindividual study.
Aim of the study was to investigate: 1) if second eye cataract surgery under topical anesthesia is more painful than surgery on the first eye, 2) if pain experienced during the procedure on the first eye may predict the pain of the second procedure, and 3) if patients' cooperation is different between the first and the second eye procedure. ⋯ After uneventful cataract surgery under topical anesthesia, pain experienced and cooperation did not differ between first and second eye procedures. A correlation was found between pain scores of the first and the second eye procedures.
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Graefes Arch. Clin. Exp. Ophthalmol. · Aug 2011
Biomechanical property analysis after corneal collagen cross-linking in relation to ultraviolet A irradiation time.
To study the biomechanical effect of riboflavin-ultraviolet A irradiation (UVA)-induced collagen cross-linking (CXL) in porcine corneas using two different exposure times of 30 and 60 min. ⋯ The application of 30-min UVA CXL treatment with riboflavin increased stiffness of the porcine corneal tissue. A 60-min UVA-radiated tissue presents lower stiffness than the 30-min treated tissue, showing a similar biomechanical behavior than the untreated corneas. A prolongation of the UVA irradiation time may cause structural weakening of the porcine corneas.
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Graefes Arch. Clin. Exp. Ophthalmol. · Aug 2011
Ocular surface squamous neoplasia in xeroderma pigmentosum: clinical spectrum and outcome.
To study the clinical spectrum and therapeutic outcome of ocular surface squamous neoplasia (OSSN) in patients with xeroderma pigmentosa (XP). ⋯ OSSN occurs predominantly in the elderly, but in patients of XP it tends to occur at a younger age (6-22 years). The disease appeared to be more aggressive (recurrence rate = 64.3%) than usual. Awareness and prompt management with close follow up is warranted in these patients.