Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine
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An automated rapid data analysis scheme for NMR spectroscopy time course studies is presented. This method uses a high signal-to-noise reference spectrum collected at the beginning of a time course as a lineshape model to analyze subsequent low signal-to-noise data collected with higher time resolution. The method is fast (approximately 1 s to evaluate two peaks in a 2K spectrum) and easily implemented on NMR spectrometer computers. An application of this method to spectroscopic studies on the heart is provided.
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Realization of 1D 1H spectra of superfused brain slices of rats is described. Two-dimensional 1H-1H COSY spectra have been used for the direct assignment of resonances on living tissue (lactate, GABA, N-acetyl aspartate, aspartate, glutamine/glutamate, creatine/phosphocreatine, taurine, inositol, choline/ethanolamine). Utilization of the SUPERCOSY sequence permits simultaneously the enhancement of off-diagonal signals for small metabolites and the reduction of the signals for water and macromolecules.
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Aggregation of human erythrocytes at physiological hematocrit by hydroxyethyl starch (HES) was investigated with a 1H nuclear magnetic resonance method which measures the erythrocyte mean water exchange time, tau a. A relationship that takes into account the effect of the aggregate as the water-exchanging unit was used to transform tau a values into the number of cells per aggregate, n. ⋯ The aggregation of erythrocytes in the presence of HES was found to follow first-order kinetics. The feasibility of this technique for application in the study of diseased states is indicated.