Journal of neurotrauma
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Journal of neurotrauma · Nov 2011
Blast-induced color change in photonic crystals corresponds with brain pathology.
A high incidence of blast exposure is a 21st century reality in counter-insurgency warfare. However, thresholds for closed-head blast-induced traumatic brain injury (bTBI) remain unknown. Moreover, without objective information about relative blast exposure, warfighters with bTBI may not receive appropriate medical care and may remain in harm's way. ⋯ With prototype BID arrays affixed to the animals, we found that BID color changes corresponded with subtle brain pathologies, including neuronal degeneration and reactive astrocytosis. These subtle changes were most notable in the dentate gyrus of the hippocampus, cerebral cortex, and cerebellum. These data demonstrate the feasibility of using a materials-based, power-free colorimetric BID as the first self-contained blast sensor calibrated to correspond with brain pathology.
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Journal of neurotrauma · Nov 2011
Comparative StudyA detailed viscoelastic characterization of the P17 and adult rat brain.
Brain is a morphologically and mechanically heterogeneous organ. Although rat brain is commonly used as an experimental neurophysiological model for various in vivo biomechanical studies, little is known about its regional viscoelastic properties. To address this issue, we have generated viscoelastic mechanical property data for specific anatomical regions of the P17 and adult rat brain. ⋯ All models fit the data equally with no significant differences between them (F-test; p>0.05). The F-test was also used to statistically determine that a Prony series with three time-dependent parameters accurately fit the data with no added benefit from additional terms. The age- and region-dependent rat brain viscoelastic properties presented here will help inform future biomechanical models of the rat brain with specific and accurate regional mechanical property data.
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Journal of neurotrauma · Nov 2011
Trauma-induced plasmalemma disruptions in three-dimensional neural cultures are dependent on strain modality and rate.
Traumatic brain injury (TBI) results from cell dysfunction or death following supra-threshold physical loading. Neural plasmalemma compromise has been observed following traumatic neural insults; however, the biomechanical thresholds and time-course of such disruptions remain poorly understood. In order to investigate trauma-induced membrane disruptions, we induced dynamic strain fields (0.50 shear or compressive strain at 1, 10, or 30?sec(?1) strain rate) in 3-D neuronal-astrocytic co-cultures (>500??m thick). ⋯ At 48?h post-insult, cell death increased significantly in the high-strain-rate group, but not after quasi-static loading, suggesting that cell survival relates to the initial extent of transient structural compromise. Cells were more sensitive to bulk shear deformation than compression with respect to acute permeability changes and subsequent cell survival. These results provide insight into the temporally varying alterations in membrane stability following traumatic loading and provide a basis for elucidating physical cellular tolerances.
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Increased intracranial pressure (ICP) caused by edema following severe traumatic brain injury (TBI) or stroke contributes to high rates of mortality and morbidity. The search continues for more effective treatments that target the edema that contributes to increased ICP. We previously described the effect of the fixed charge density (FCD) of brain on its swelling behavior according to the Donnan effect. ⋯ ChABC reduced swelling in live slices of tissue even within the first 2?h following dissection. It also significantly reduced the FCD, initial tissue swelling, and volume change in response to hypotonic bathing solution in porcine cortical brain tissue. The use of ChABC to reduce tissue FCD may be an effective method for reducing brain edema and controlling ICP following injury.
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Journal of neurotrauma · Nov 2011
Comparative StudyStrain-based regional traumatic brain injury intensity in controlled cortical impact: a systematic numerical analysis.
Regional strain-based brain injury intensity during controlled cortical impact (CCI) was studied using a three-dimensional numerical rat brain model. A full factorial design of CCI computer experiments was performed using two typical impactor shapes (flat or hemispherical) at a fixed impact velocity of 4?m/s with various impact depths (1, 1.5, 1.6, 2, 2.5, 2.7, and 3?mm) and various impactor diameters (4, 5, 6, 8, and 9.5?mm). In total, 70 CCI cases were simulated numerically. ⋯ For the flat impactor group, the 5?mm diameter impactor induced more tissue strain in the corpus callosum/hippocampus, and a smaller impactor induced more strain in the thalamus. For the hemispherical impactor group, a larger impactor tended to induce more tissue strain in subcortical regions, with the exception of the 6?mm diameter impactor. This study systematically predicts regional intensity of primary brain injury according to tissue strain distributions in the hope that strain distribution maps may become a common platform to compare CCI severities with different configurations.