Journal of investigative medicine : the official publication of the American Federation for Clinical Research
-
The present study aimed to investigate the influence of several important preanalytical factors (storage period of the tumor block, maximal diameter of the tumor circled area, tumor volume and tumor fraction) on the isolated DNA from formalin-fixed paraffin-embedded (FFPE) tissues in a series of thyroid carcinomas. ⋯ The maximal diameter of the tumor circled area, tumor volume and tumor fraction are important morphological factors that correlate with the DNA concentration and should be carefully assessed in routine practice prior to performing DNA isolation from FFPE tissues.
-
Gastric cancer (GC) is a commonly diagnosed malignancy with a high mortality rate worldwide. Despite advances in therapeutic approaches, the 5-year survival rate of patients with GC remains poor. A type of non-coding RNA, circular RNA (circRNA), has been discovered. ⋯ Real-time PCR experiments on hsa_circ_0103398 and hsa_circ_0127859 in tumors and normal tissues showed that these circRNAs were significantly upregulated in GC. High expression of hsa_circ_0103398 and hsa_circ_0127859 was closely associated with unfavorable survival time. Our findings indicated that a 5-circRNA signature might serve as a candidate prognostic biomarker of GC.
-
The present study aimed to analyze the methylation pattern of the MIR200 family in the colorectal tissues and peripheral blood of colorectal cancer (CRC) patients. Previous informed consent, 102 samples of colorectal tissues (tumor and adjacent normal tissues) and 40 peripheral blood samples were collected from CRC patients. Additionally, we included a reference group of 40 blood samples. ⋯ MIR200B/MIR200A/MIR429 methylation analysis discloses no differences among tissues (p>0.05). However, MIR200C/MIR141 methylation showed differences between colorectal tissues and peripheral blood of CRC patients (p<0.0001) and mainly methylated alleles were observed in peripheral blood. These findings suggest a tissue-specific methylation pattern for the MIR200C/MIR141 promoter.
-
In some patients diabetic ketoacidosis (DKA) causes acute endothelial injury and multiorgan failure. Measurement of glycosaminoglycan (GAG) and advanced glycation end products (AGE) could provide information to help understand the biochemical events associated with poor outcomes in these patients. This study included 37 patients with DKA admitted to an intensive care unit. ⋯ There were no differences in soluble receptor for AGE levels or GAG levels between the control subjects and patients with DKA. Patients with DKA had lower circulating levels of AGE and glycated albumin than control subjects. These results may reflect absorption of these proteins to damaged capillary surfaces or loss of proteins into interstitial spaces secondary to increased endothelial permeability.
-
In this study, we aimed to explore the potential involvement of miR-3150b in hepatocellular carcinoma (HCC) carcinogenesis. ⋯ MiR-3150b suppressed HCC cell proliferation, invasion and migration by targeting GOLPH3.