Pulmonary pharmacology & therapeutics
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Pulm Pharmacol Ther · Jan 2004
Inhaled nitric oxide exacerbated phorbol-induced acute lung injury in rats.
In this study, we determined the effect of inhaled nitric oxide (NO) on the acute lung injury induced by phorbol myristate acetate (PMA) in isolated rat lung. Typical acute lung injury was induced successfully by PMA during 60 min of observation. PMA (2 microg/kg) elicited a significant increase in microvascular permeability, (measured using the capillary filtration coefficient Kfc), lung weight gain, lung weight/body weight ratio, pulmonary arterial pressure (PAP) and protein concentration of the bronchoalveolar lavage fluid. ⋯ In addition, L-NAME (5 mM) significantly attenuated PMA-induced acute lung injury except for PAP. These experimental data suggest that inhaled NO significantly exacerbated acute lung injury induced by PMA in rats. L-NAME attenuated the detrimental effect of inhaled NO.
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Pulm Pharmacol Ther · Jan 2004
Inhaled milrinone for the improvement of contractility of fatigued diaphragm in dogs: a dose-ranging study.
The present study was undertaken to evaluate the efficacy of inhaled milrinone on contractility of fatigued diaphragm in dogs. Animals were divided into four groups of seven each. In each group, diaphragmatic fatigue was induced by intermittent supramaximal bilateral electrophrenic stimulation at a frequency of 20 Hz applied for 30 min. ⋯ The increase in Pdi was more in Group IV than in Group III (P<0.05). Compared with Group I, Pdi to each stimulus did not change in Group II. In conclusion, inhaled milrinone, in doses more than 0.2 mg/ml, is effective for improving contractility of fatigued diaphragm in dogs.