Critical care : the official journal of the Critical Care Forum
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Comparative Study
Assessment of tissue oxygen tension: comparison of dynamic fluorescence quenching and polarographic electrode technique.
Dynamic fluorescence quenching is a technique that may overcome some of the limitations associated with measurement of tissue partial oxygen tension (PO2). We compared this technique with a polarographic Eppendorf needle electrode method using a saline tonometer in which the PO2 could be controlled. We also tested the fluorescence quenching system in a rodent model of skeletal muscle ischemiahypoxia. ⋯ Measurement of tissue PO2 using fluorescence quenching is at least as accurate as measurement using the Eppendorf needle electrode in vitro, and may prove useful in vivo for assessment of tissue oxygenation.
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Comparative Study
ICUs worldwide: an overview of critical care medicine in South Africa.