Pak J Med Sci
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To investigate the clinical effect of posterior nasal nerve combined with anterior ethmoid neurotomy in the treatment of moderate to severe persistent allergic rhinitis. ⋯ Posterior nasal nerve combined with anterior ethmoid neurotomy is a safe and reliable surgical method for the treatment of moderate to severe persistent allergic rhinitis, boasting a variety of benefits such as symptomatic relief, improved quality of life, and reduced adverse reactions, which is worthy of clinical promotion.
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Polymorphism in cytochrome P450 3A4 (CYP3A4) and its regulatory gene peroxisome proliferator activated receptor (PPARA) may significantly affect the metabolism of tacrolimus. This study aims to explore the effect of the single nucleotide polymorphisms (SNP) of cytochrome P450 3A4 and PPARA on the pharmacological variables of adverse effects and the concentration-to-dose ratio (CDR) of the immune suppressant drug tacrolimus in Pakistani liver transplant recipients. ⋯ Genotyping for CYP3A4 rs35599367 polymorphism during dose titration may shorten the duration to reach optimal tacrolimus trough levels and may help predict adverse events in transplant recipients receiving tacrolimus; genotyping for PPARA rs4253728 and rs4823613 may predict the incidence of ACR.
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Extraction of DNA and RNA is the first step in genomics and transcriptomics studies. Phenol-chloroform method for DNA extraction has been the widely used method. However, this method is relatively expensive and time-consuming. The objective of the present study was to validate a cost and time-effective protocol that will reduce the burden of molecular biology-based research and make a difference in laboratories with limited resources. ⋯ The TRIzol™ method for RNA and DNA co-extraction is fast, simple and economical technique. So, it can be adopted for routine molecular biology analyses in limited resources setup.
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To observe the changes of blood gas and serum indexes in patients with acute heart failure complicated with respiratory failure treated by noninvasive ventilator-assisted ventilation. ⋯ In patients with acute heart failure and respiratory failure, noninvasive ventilator-assisted ventilation results in improved blood gas analysis indexes and lower levels of heart disease markers, NT-pro BNP and cTnI.
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To determine the frequency of various hematological manifestations among newly diagnosed cases of pulmonary tuberculosis on complete blood counts. ⋯ Hematological parameters like high Erythrocyte Sedimentation Rate (ESR) and anemia were commonly detected in newly diagnosed patients with pulmonary tuberculosis. Thrombocytosis was seen more commonly in male patients of pulmonary tuberculosis.