The Journal of investigative dermatology
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J. Invest. Dermatol. · Sep 1980
Immunofluorescence analysis of collagen, fibronectin, and basement membrane protein in scleroderma skin.
Scleroderma skin and the subcutaneous tissue was studied by indirect immunofluorescence with specific antibodies against interstitial collagens and procollagens, against fibronectin and against the basement membrane proteins Type IV collagen and laminin. Staining for Type I procollagen and fibronectin was distinctly increased in the lower dermis and subcutaneous tissue. When compared with normal skin the data suggests that fibrosis may start around capillaries and in close proximity to adipose cells. Additional changes in the distribution to Type IV collagen and laminin were found in some patients and probably reflect the alterations in small blood vessels.
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J. Invest. Dermatol. · Jun 1979
Experimental tinea versicolor in rabbits and humans with Pityrosporum orbiculare.
The purpose of this investigation was to produce experimental tinea versicolor in rabbits and humans with Pityrosporum orbiculare. Inoculation with P. orbiculare under plastic occlusion on the glabrous follicle-rich inside of the rabbit ear resulted in a tinea versicolor-like lesion after 1 week in 3 of 4 animals. ⋯ It was not possible to produce experimental infections without occlusion. Spontaneous healing usually occurred.
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J. Invest. Dermatol. · Oct 1978
Comparative StudyThe evaluation of topical anti-inflammatory activity on rat ears subjected to thermal injury.
Topical anti-inflammatory activity of steroidal and non-steroidal agents was assessed on inflammation produced by heat. A burn was produced on the ears of rats and the inflammation was quantitated gravimetrically. ⋯ Cholesterol, a steroid devoid of anti-inflammatory activity, was inactive in this test. Hydrocortisone acetate, in particular, appears to be less effective in inhibiting this type of inflammation than inflammation produced by croton oil.
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J. Invest. Dermatol. · Oct 1978
Comparative StudyViral immune complexes in systemic lupus erythematosus: C-type viral complex deposition in skin.
Punch biopsies were examined by indirect immunofluorescence for immune complex deposits containing C-type viral antigen. Antisera specific for immunoglobulins and HEL-12 virus mediated fluorescence at the dermal-epidermal junction and in vessel walls of 16 of 16 biopsies involved skin from patients with systemic lupus erythematosus (SLE). ⋯ Ten biopsies from uninvolved skin of the patients with SLE did not react with the antiviral serum, nor did tissue from 9 patients with discoid lupus erythematosus, psoriasis, bullous pemphigoid or normal skin. These data support the hypothesis that C-type viral immune complexes participate in the pathogenesis of SLE.
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J. Invest. Dermatol. · Dec 1976
Immunofluorescent localization of basement membrane in lesions of dermatitis herpetiformis.
Dermatitis herpetiformis (DH) is a blistering disease with a characteristic histology that includes papillary edema, neutrophilic papillary microabscesses, and development of subepidermal blisters. In spite of this pathologic sequence occurring entirely beneath the basement membrane zone, prior studies have indicated that the basement membrane, as defined by period acid-Schiff (PAS) or silver stains, lies at the floor of fully formed blisters or is destroyed by the disease process. To more accurately assess its location in primary lesions of DH, the basement membrane was stained using immunofluorescent techniques. ⋯ PAS stains of the same or serial sections show the basement membrane to be in the roof or at the floor of the blisters. PAS stains of sections from formalin-fixed lesional skin, on the other hand, show the basement membrane to routinely lie at the blister floor, when not destroyed. The BP-stained epidermal basement membrane has greater anatomic and functional significance than either the PAS-or silver-stained basement membrane for two reasons: (1) it corresponds to a specific morphologic structure, the lamina lucida, a part of the epidermis, and remains attached to the rest of the epidermis unless destroyed; and (2) it is antigenic, capable of binding with BP antibodies.