Voprosy virusologii
-
Voprosy virusologii · May 2014
[Genetic characterization of the Sakhalin virus (SAKV), Paramushir virus (PMRV) (Sakhalin group, Nairovirus, Bunyaviridae), and Rukutama virus (RUKV) (Uukuniemi group, Phlebovirus, Bunyaviridae) isolated from the obligate parasites of the colonial sea-birds ticks Ixodes (Ceratixodes) uriae, White 1852 and I. signatus Birulya, 1895 in the water area of sea of the Okhotsk and Bering sea].
Full-length genomes of the Sakhalin virus (SAKH) and Paramushir virus (PRMV) (Sakhalin group, Nairovirus, Bunyaviridae) isolated from the ticks Ixodes uriae White 1852 were sequenced using the next-generation sequencing (Genbank ID: KF801659, KF801656). SAKV and PRMV have 81% identity for the part of RNA-dependent RNA-polymerase (RdRp) on the nucleotide level and 98.5% on the amino acid level. Full-length genome comparison shows that SAKV have, in average, from 25% (N-protein, S-segment) to 50% (RdRp, L-segment) similarity with the nairoviruses. ⋯ RUKV is closely related (93.0-95.5% similarity) with our previously described Komandory virus (KOMV). RUKV and KOMV form separate phylogenetic line neighbor of Manawa virus (MWAV) isolated from the ticks Argas abdussalami Hoogstraal et McCarthy, 1965 in Pakistan. The value of the similarity between RUCV and MWAV is 65-74% on the amino acid level.
-
Voprosy virusologii · Mar 2014
[Taxonomy of previously unclassified Tamdy virus (TAMV) (Bunyaviridae, Nairovirus) isolated from the Hyalomma asiaticum asiaticum Schülce et Schlottke, 1929 (Ixodidae, Hyalomminae) in the Middle East and transcaucasia].
Complete genome sequencing of three Tamdy (TAMV) virus strains was carried out. The prototype strain TAMV/LEIV-1308Uz was isolated for the very first time from the Hyalomma asiaticum asiaticum Schülce et Schlottke, 1929 (Ixodidae, Hyalomminae) collected in the August 1971 from sheep in the arid area near Namdybulak town (41 degrees 36' N, 64 degrees 39' E) in the Tamdinsky district of the Bukhara region (Uzbekistan). TAMV was revealed to be a prototype member of the new phylogenetic group within the limits of the Nairovirus. ⋯ The homology between the TAMV/LEIV-1308Uz and TAMV/LEIV-10226Az for GnGc is 93%. The TAMV/LEIV-6158Ar has 90% homology for this protein with the TAMV/LEIV-1308Uz and 93% with the TAMV/LEIV-10226Az, respectively. Differences in nucleocapsid protein between three TAMV strains are 5-7%.
-
Voprosy virusologii · Jan 2014
[Genetic characterization of the Geran virus (GERV, Bunyaviridae, Nairovirus, Qalyub group) isolated from the ticks Ornithodoros verrucosus Olenev, Zasukhin and Fenyuk, 1934 (Argasidae) collected in the burrow of Meriones erythrourus Grey, 1842 in Azerbaijan].
The full-length genome of the unclassified Geran virus (GERV, strain LEIV-10899Az) isolated from the ticks (Ornithodoros verrucosus Olenev, Zasukhin and Fenyuk, 1934 (Argasidae, Ornithodorinae)) collected in the burrow of the red-tailed gerbils (Meriones (Cricedidae) erythrourus Grey, 1842) near the Geran station (Azerbaijan) was sequenced using the next-generation approach (GenBank ID: KF801649). It was shown that the GERV is a new representative of the Nairovirus genus (family Bunyaviridae). ⋯ Comparing the GERV with the QYBV sequences (partial sequence 413 n.o. of RdRp gene) revealed a high level of homology: 74.3 and 97.4% for the nucleotide and amino acid sequences, respectively. The data obtained in this work provided an opportunity to classify the GERV to the QYBV group; the Nairovirus genus, to the family Bunyaviridae.
-
Voprosy virusologii · Jan 2014
[Genetic characterization of the Zaliv Terpeniya virus (ZTV, Bunyaviridae, Phlebovirus, Uukuniemi serogroup) strains isolated from the ticks Ixodes (Ceratixodes) uriae White, 1852, obligate parasites of the Alcidae birds, in high latitudes of Northern Eurasia and the mosquitoes Culex modestus Ficalbi, 1889, in subtropics Transcaucasus].
Complete genome sequences were obtained for the LEIV-13841Ka (ID GenBank KF767463-65) and LEIV-279Az (ID GenBank KF767460-62) virus strains, which were classified as different strains of the Zaliv Terpeniya virus (ZTV). LEIV-13841Ka was isolated from the ticks Ixodes (Ceratixodes) uriae White, 1852 collected on Ariy Kamen (Commander Islands) in 1986. LEIV-279Az was isolated from the mosquitoes Culex modestus Ficalbi, 1889, collected in heron colony (Ardea Linnaeus, 1758) in Azerbaijan (1969) and was initially identified as Uukuniemi virus (UUKV). ⋯ NP proteins of ZTV/LEIV-13841Ka and LEIV-279Az have 88.7% and 84.6% homologies with ZTV/LEIV-271Ka both for amino acid and nucleotide sequences, respectively. Thus, for the very first time we demonstrated ZTV strain isolated from mosquitoes in subtropical Transcaucasia zone. Obtained results permit to expand suggested areal of ZTV and to fill up data upon the ecology of the Uukuniemi virus group.
-
Voprosy virusologii · Nov 2013
[Genetic characterization of new Komandory virus (KOMV; Bunyaviridae, Phlebovirus) isolated from the ticks Ixodes uriae, collected in guillemot (Uria aalge) nesting sites on Komandorski islands, the Bering sea].
Unclassified virus named Komandory virus (KOMV) isolated in Komandorski islands from the ticks Ixodes uriae in 1986 was partially sequenced. The KOMV nucleocapsid (N) protein sequence shows 30-40% identity level with the mosquito-borne phleboviruses and 43% with the Uukuniemi virus (UUKV) group (Phlebovirus, Bunyaviridae). ⋯ The KOMV RdRp identity with the Manawa virus reaches 74%, while showing 63% level at average with the other UUKV group viruses. According to the results of molecular-genetic and phylogenetic analysis, the KOMV is a new member of the UUKV group (Phlebovirus, Bunyaviridae).