Voprosy virusologii
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Voprosy virusologii · Nov 2013
[The taxonomy of the Baku virus (BAKV; Reoviridae, Orbivirus) isolated from the birds obligate parasites Argasidae ticks in Azerbaijan, Turkmenistan, and Uzbekistan].
The Baku virus (BAKV) was originally isolated from the ticks Ornithodoros capensis Neumann, 1901 (Acari: Argasidae) collected from the seagull (Larus argentatus) seating nests on the islands of the Baku archipelago, the Caspian sea. BAKV was assigned to Kemerovo group (KEMV) (Orbivirus, Reoviridae). The BAKV was frequently isolated from the ticks O. coniceps Canestrini, 1980, collected from L. argentatus and tern (Sterna hirundo) nests in Turkmenia and pigeon (Columba livia neglecta) nests in Uzbekistan. ⋯ The maximum identity level of the T2 protein (64.8%) is observed for the tick-borne viruses of the GIV (KEMV) group. According to the conducted molecular-genetic and phylogenetic analysis, the BAKV is a novel species of the genus Orbivirus. It forms a phylogenetic group distinctly related to the GIV group.
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Voprosy virusologii · Sep 2013
[The taxonomy of the Khasan virus (KHAV), a new representative of phlebovirus genera (Bunyaviridae), isolated from the ticks haemaphysalis longicornis (Neumann, 1901) in the Maritime Territory (Russia)].
The Khasan virus (KHAV) was originally isolated in Khasansky District and Maritime Territory in 1971 from the ticks Haemophysalis longicornis (Neumann, 1901) collected from the deers Cervus nippon (Temmink, 1838). Based on the biological properties and virion morphology, KHAV was identified as an unclassified member of the Bunyaviridae family. In order to elucidate the KHAV taxonomy in more detail, viral genome was partially sequenced using the next-generation sequencing technology. ⋯ KHAV is phylogenetically mostly related to the viruses of the Uukuniemi group and has up to 62% identity with them. The maximum identity level is observed for sequences of the RNA-dependent-RNA-polymerase (RdRp) gene. The KHAV homology level with the tick-borne Uukuniemi group viruses is 50 to 62%; however, for the mosquito-borne phleboviruses it does not exceed 30%.
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Voprosy virusologii · Jul 2013
[Molecular-genetic characterization of the Bhanja virus (BHAV) and the Razdan virus (RAZV) (Bunyaviridae, Phlebovirus) isolated from the Ixodes ticks Rhipicephalus bursa (Canestrini and Fanzago, 1878) and Dermacentor marginatus (Sulzer, 1776) in transcaucasus].
Two bunyaviruses, Bhanja (BHAV, LEIV-Az1818) isolated from the Ixodes ticks Rhipecephalus bursa (Canestrini and Fanzago, 1878) in Azerbaijan (1973) and Razdan (RAZV; strain LEIV-Arm2741) isolated from the Dermacentor marginatus (Sulzer, 1776) ticks in Armenia (1972), were de novo sequenced (on the Illumina platform). The amino acid identity between these viruses proteins were 95.8% (RdRp, L-segment), 90.3% (GnGc, M-segment), and 92.5% (N, S-segment). Thus, RAZV was classified to BHAV group. ⋯ BHAV LEIV-Az1818 is most closely related to the Indian strain BHAV IG690 (99%), while showing 90% identity with the European BHAV isolates. The genome structure of BHAV and RAZV is typical of the tick-transmitted phleboviruses. Based onthe result of the molecular-genetic and phylogenetic analysis RAZV has been classified as belonging to BHAVgroup in the genus Phlebovirus (Bunyaviridae).
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Voprosy virusologii · May 2011
[Monoclonal antibodies with high virus-neutralizing activity against pandemic influenza virus A/llV-Moscow/01/2009 (H1N1)swl].
The authors have obtained a panel of 7 monoclonal antibodies (MAbs) against pandemic influenza virus A/IIV-Moscow/01/2009 (HIN1)swl isolated in Russia. One MAb is directed to a NP protein linear epitope and interacts with all the influenza A viruses under study. ⋯ MAbs differentiate pandemic influenza viruses A(H1N1)swl from seasonal influenza A(H1N1), A(H3N2), and B viruses. The high neutralizing activity of MAbs permits their use to study the fine antigen structure of influenza virus hemagglutinin and to differentiate the A(H1N1) pandemic influenza viruses and offers promise for obtaining humanized antibodies in order to make specific prevention and treatment of influenza.
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Voprosy virusologii · Sep 2010
[Molecular genetic studies of the susceptibility of epidemic influenza A(H1N1) virus strains isolated in the 2006-2009 seasons in Russia to oseltamivir (Tamiflu)].
Oseltamivir (Tamiflu) is recommended by WHO experts as a drug to treat and prevent of influenza and to create stocks if its new pandemic variant occurs. The susceptibility of influenza viruses to oseltamivir was studied by polymerase chain reaction-based techniques detecting specific mutations in the neuraminidase gene. The increase in the number of oseltamivir-resistant influenza viruses, isolated from the Russian Federation, with type 1 neuraminidase H274Y mutation from 49% (2007-20008) to 92% (2008-2009) did not depend on the frequency of oseltamivir use. Full correlation of the results obtained by various techniques allows them to be used to monitor the susceptibility of influenza viruses to oseltamivir.