Journal of hygiene, epidemiology, microbiology, and immunology
-
J Hyg Epidemiol Microbiol Immunol · Jan 1987
In vitro effects of organic solvents on immunity indicators in serum.
Serum treatment in vitro with organic solvents (chloroform, ether, toluene) failed to produce an effect on immunoglobulin levels and activity. After chloroform and ether treatment, no complement activity could be determined, with chloroform-treated serum beginning to express anticomplement activity against autologous, allogenic and xenogenic sera. ⋯ Toluene did not influence any of the parameters tested, while ether blocked complement activity without affecting either the concentration or activity of the other components under investigation. The obtained findings are discussed from the aspect of organic solvent applications in preparing immune products and determining immunity indicators in the serum or other biological fluids.
-
J Hyg Epidemiol Microbiol Immunol · Jan 1986
Case ReportsA case of human cryptosporidiosis in Czechoslovakia.
A case of human cryptosporidiosis, the first one reported in Czechoslovakia, is described. The disease was diagnosed by the presence of Cryptosporidium oocysts in the feces. The methods used independently to identify oocysts were the fecal flotation technique employing a saturated solution of sucrose and the microscopic examination of stained fecal smears. ⋯ Excretion positivity for Cryptosporidium oocysts in the feces was detectable at 3 and 5 days after appearance of first clinical manifestations. Bacteriological examination was repeatedly negative. This finding leaves little doubt as to existence of human cryptosporidiosis in Czechoslovakia, but what remains obscure is its overall contribution to the etiology of diarrheal diseases encountered in the population.
-
J Hyg Epidemiol Microbiol Immunol · Jan 1984
Comparative StudyTo the etiology of whooping cough syndrome. Part I: Bacteriological examinations.
A total of 7530 cases of whooping cough syndrome was bacteriologically examined from January 1978 through June 1983. Bordetella pertussis was confirmed by cultivation in 19, B. parapertussis in 284 and H. influenzae in 285 examinees. ⋯ Routine bacteriological screenings conducted during the same time period among the preschool children from Prague 10 (a total of 3651 children of 4-6 years of age were examined after the first or before the second revaccination) gave no positive isolation of B. pertussis, isolates of B. parapertussis and H. influenzae were obtained from 43 and 107 children, respectively. This comprehensive bacteriological examination helped identify the causative agent of whooping cough syndrome and reduced thus somewhat the number of cases of uncertain etiology.
-
J Hyg Epidemiol Microbiol Immunol · Jan 1983
Hygienic evaluation of repurification schemes for waste waters containing complexes for organic substances.
Sanitary-chemical and sanitary-toxicological methods were used to study two repurification schemes for biologically purified waste waters from a petrochemical industrial complex. These repurification schemes were, (1) filtration through quartz sand, adsorption to activated charcoal, chlorination; (2) coagulation, filtration through quartz sand, adsorption to activated charcoal, chlorination. ⋯ Provided that the corresponding sanitary norms are observed, the use of repurification Scheme 1 would be economically reasonable where repurified waste water is recirculated in the industrial plant. Repurification Scheme 2 is recommended where purified waste water is disposed into low-capacity reservoirs.
-
J Hyg Epidemiol Microbiol Immunol · Jan 1980
Development of methodological approaches to the determination of safe levels of activity of harmful substances in the air of the working environment.
1. An experiment study was carried out to substantiate fundamental approaches to the determination of shift-average and maximum instantaneous concentration of highly cumulative substances in the air of the working environment. 2. The determination of maximum instantaneous concentrations in the air of the working environment is maintained for some groups of chemical compounds (irritant poisons, substances having acute effect, etc). An express, method of determining MAC in the air of the working environment has been developed for the purpose of hygienic norm-setting for irritant substances.