Sheng wu gong cheng xue bao = Chinese journal of biotechnology
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Sheng Wu Gong Cheng Xue Bao · Jul 2011
[Preface for special issue on industrial biotechnology. Preface].
Industrial biotechnology, which employs microorganisms or enzymes to produce industrial useful products, has been considered as a promising solution for the sustainable development of society and economy. This special issue collects some recent research progresses on industrial biotechnology in China, including research articles in the field of genetic engineering, metabolic engineering and synthetic biology, physiological engineering, fermentation engineering and biochemical engineering, biocatalysis and biotransformation, as well as new biotechniques and methods.
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Sheng Wu Gong Cheng Xue Bao · Jul 2001
[Effects of fungal elicitor on cell status and taxol production in cells suspension cultures of Taxus chinesis var. mairei].
Effects of fungal elicitor on cell redox status and taxol production were studied in suspension cultures of Taxus chinesis var. mairei in the late exponential stage. The results show that fungal elicitor induced oxygen burst, changes of the cell redox status, alkalinization of medium and the fluctuation of the activity of redox enzymes with a sequence. ⋯ The activity of CAT was inhibited at first and followed by an obvious increase, while the activity of PAL was promoted. The taxol yield was 5 folds of the control, reaching to 67 mg/L.
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Sheng Wu Gong Cheng Xue Bao · Mar 2001
[Effect of dissolved oxygen on the suspension culture of Taxus chinensis].
The effect of dissolved oxygen controlled level on the taxol production by suspended cell culture of Taxus chinensis was examined in shake flasks and in a 20L aerated and agitated bioreactor with a six-flat-bladed turbine. It was found in shake flasks that oxygen supply exhibited significant influence on the production of taxol. ⋯ Moreover, taxol accumulation was remarkably improved by the cultivation of cells initially at the DO level of 60% during the growth phase for 14 days followed by changing the DO level to 20%-40% during the taxol production phase for another 10 days. An optimum taxol level of 7.2 mg/L was obtained by the dissolved oxygen controlled process.