Articles: general-anesthesia.
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The Journal of physiology · Jun 1971
The imprecision arising from the application of the Henderson-Hasselbalch relationship to the blood of anesthetized dogs.
1. Arterial blood samples were obtained from anaesthetized dogs in which either a respiratory alkalaemia, a respiratory acidaemia or a non-respiratory acidaemia was produced and from animals in which no deliberate changes in acid-base balance were produced. The samples were analysed for pH, carbon dioxide tension and concentration, oxygen tension and concentration and haematocrit. ⋯ The values of pK' increased with an alkalaemia and decreased with an acidaemia.4. In addition to the variation of pK' with pH there was a considerable residual scatter of pK' values at any one pH value.5. It was concluded that in these experimental conditions the use of the Henderson-Hasselbalch equation in a form not dealing with activities was unacceptable and could lead to much larger errors than has hitherto been thought.