• Singap Med J · Apr 2020

    Generation of IgE-specific cytotoxic T lymphocytes as a novel immunotherapeutic approach for the treatment of allergic asthma.

    • Kangle Cui, Yanna Chen, Li Zhou, Chunxia Yang, Yao Du, Weixing Shi, Zeling Cai, and Jian Chen.
    • College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China.
    • Singap Med J. 2020 Apr 1; 61 (4): 212-220.

    IntroductionOverproduction of immunoglobulin E (IgE) by a subset of B cells plays a key role in the pathogenesis of allergic asthma. Anti-IgE monoclonal antibodies have been successfully used to treat the disease, but long-term application is required.MethodsFor this study, cytotoxic T lymphocytes (CTLs) against IgE-producing B cells were generated ex vivo by stimulating naive CD8 T cells with IgE-derived peptides presented by Drosophila-derived artificial antigen-presenting cells. Based on the treatment of allergic asthma in mice, the inhibitive effect of this CTL on IgE responses and airway inflammation was determined with the enzyme-linked immunosorbent assay and histochemical method.ResultsThe IgE-specific CTLs effectively lysed target cells in vitro, while the adoptively transferred CTLs specifically inhibited IgE responses and airway inflammation in an asthmatic mouse model. The effect of IgE-specific CTLs is MHC (major histocompatibility complex) Class I-restricted and requires the expression of perforin.ConclusionIgE-specific CTLs generated ex vivo may provide a novel treatment for allergic asthma and lead to a new therapy for other immunological disorders.Copyright: © Singapore Medical Association.

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