• Eurosurveillance · Apr 2020

    An alternative workflow for molecular detection of SARS-CoV-2 - escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020.

    • Anna S Fomsgaard and Maiken Worsøe Rosenstierne.
    • Department of Virus and Microbiological Special Diagnostics, Statens Serum Institut, Copenhagen, Denmark.
    • Eurosurveillance. 2020 Apr 1; 25 (14).

    AbstractThe World Health Organization has declared COVID-19 caused by the newly discovered SARS-CoV-2 a pandemic. Due to growing demand for reagents and/or kits to extract SARS-CoV-2 RNA for subsequent RT-qPCR diagnostics, there is a worldwide risk of shortages. With a detection sensitivity of 97.4% (95% CI: 86.2-99.9%), we describe a simple, fast, alternative workflow for molecular detection of SARS-CoV-2, where samples are simply heat-processed for 5 min at 98 °C before a commonly-used RT-qPCR procedure.

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