• Leopoldo Santos-Argumedo, Laura Berrón-Ruiz, Gabriela López-Herrera, and Nidia Carolina Moreno-Corona.
• Instituto Politécnico Nacional, Center of Research and Advanced Studies, Department of Molecular Biomedicine, Mexico City, Mexico.
• Gac Med Mex. 2020 Jan 1; 156 (3): 194-200.

BackgroundAntibody deficiencies encompass a wide spectrum of pathologies and constitute approximately 50 % of primary immunodeficiencies; with cytometry, it is possible to evaluate the immune status rapidly, effectively and at low cost.ObjectiveTo assess, by means of flow cytometry, the cells of patients with three types of primary humoral immunodeficiencies.MethodUsing flow cytometry, blood samples from patients and healthy subjects were analyzed with different monoclonal antibodies.ResultsUsing various stains, a severe decrease in B lymphocytes was shown in patients with X-linked agammaglobulinemia, as well as a lack of CD154 expression in patients with hyper-immunoglobulin M syndrome, and heterogeneity of B lymphocyte subpopulations in patients with common variable immunodeficiency.ConclusionFlow cytometry enables early diagnosis of primary immunodeficiencies with a high level of confidence and, in many cases, identification of the genes involved.Copyright: © 2020 Permanyer.

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