• Helen Nazareth Veloso Dos Santos, Eduardo Ferracioli-Oda, Thaisa Silveira Barbosa, Camila Sayuri Vicentini Otani, Tatiana Tanaka, Luciane de Carvalho Sarahyba da Silva, Guilherme de Oliveira Lopes, Andre Doi, Carlos Eduardo Hirata, and Joyce Hisae Yamamoto.
• Servico de Uveites, Departmento de Oftalmologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BR.
• Clinics (Sao Paulo). 2020 Jan 1; 75: e1498.

ObjectiveTo evaluate the role of intraocular fluid analysis as a diagnostic aid for uveitis.MethodsTwenty-eight samples (27 patients including 3 HIV-infected patients) with active (n=24) or non-active (n=4) uveitis were submitted to aqueous (AH; n=12) or vitreous humor (VH) analysis (n=16). All samples were analyzed by quantitative PCR for herpes simplex virus (HSV), varicella zoster virus (VZV), cytomegalovirus (CMV), Epstein-Barr virus (EBV) and Toxoplasma gondii.ResultsThe positivity of the PCR in AH was 41.7% (5/12), with 50% (2/4) in immunocompetent and 67% (2/3) in HIV+ patients. The positivity of the PCR in VH was 31.2% (5/16), with 13% (1/8) in immunocompetent and 50% (4/8) in immunosuppressed HIV negative patients. The analysis was a determinant in the diagnostic definition in 58% of HA and 50% of VH.ConclusionEven in posterior uveitis, initial AH analysis may be helpful. A careful formulation of possible clinical diagnosis seems to increase the chance of intraocular sample analysis being meaningful.

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