• Handan Derebaşınlıoğlu, Anil Demİröz, Yağmur Aydin, Hakan Ekmekçi, Özlem Balci Ekmekçi, Övgü Aydin, and Levent Cankorkmaz.
• Department of Plastic Reconstructive and Aesthetic Surgery, Faculty of Medicine, Sivas Cumhuriyet University, Sivas, Turkey
• Turk J Med Sci. 2020 Oct 22; 50 (6): 1523-1534.

Background/AimThe aim of the study was to evaluate the protective effect of Botulinum A toxin injection against ischemia-reperfusion injury.Materials And MethodsThirty-two Sprague-Dawley rats were divided into: control, ischemia-reperfusion, ischemic preconditioning, and botulinum groups. In all groups the musculocutaneous pedicle flap was occluded for 4 h, and then reperfused to induce ischemia-reperfusion injury. Serum and tissue myeloperoxidase (MPO) and nitric oxide (NO) levels were measured at 24 h and at 10 days.ResultsTissue MPO levels did not differ significantly between the ischemic preconditioning and botulinum groups at 24 h but was significantly lower in the botulinum group at 10 days. Tissue NO levels were significantly higher in the ischemic preconditioning group compared to the botulinum group at 24 h and at 10 days. Serum MPO showed no significant difference between these two groups at 24 h but was significantly lower in the ischemic preconditioning group compared to the botulinum group at 10 days. Serum NO levels were not significantly different at 24 h but significantly higher in the botulinum group at 10 days.ConclusionFindings show that botulinum has a protective effect against the ischemia-reperfusion injury via increased NO and decreased MPO levels in tissue. Based on tissue NO levels, ischemic preconditioning was significantly higher than botulinum.This work is licensed under a Creative Commons Attribution 4.0 International License.

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