• Michiharu Sakamoto, Naoki Morimoto, Shuichi Ogino, Chizuru Jinno, Atsushi Kawaguchi, Katsuya Kawai, and Shigehiko Suzuki.
• From the *Department of Plastic and Reconstructive Surgery, Kyoto University Graduate School of Medicine, Kyoto; †Department of Plastic and Reconstructive Surgery, Kansai Medical University, Osaka; ‡Department of Biomedical Statistics and Bioinformatics, Kyoto University Graduate School of Medicine, Kyoto, Japan.
• Ann Plast Surg. 2016 Jun 1; 76 (6): 652-8.

ObjectiveThe manual application of hot water or hot metal to an animal's skin surface is often used to prepare burn wound models. However, manual burn creation is subject to human variability. We developed a new device that can control the temperature, time, and pressure of contact to produce precise and reproducible animal burn wounds and investigated the conditions required to prepare various burn wounds using our new device.MethodsWe prepared burn wounds on F344 rats using 3 contact times 2, 4, and 10 seconds using a stamp heated to 80°C. We observed the wound-healing process macroscopically and histologically and evaluated the burn depth using a laser speckle contrast-imaging device, which evaluated the blood flow of the wound.ResultsThe changes in the burned area over time, tissue perfusion of the burn wounds, histological evaluation of the burn depth by hematoxylin-eosin and azocarmine and aniline blue staining, and the epithelialization rate (the ratio of the epithelialized area to the wound length) were evaluated on histological sections. Results indicated that the burn wounds prepared with contact times of 2, 4, and 10 seconds corresponded to superficial dermal burns, deep dermal burns, and full-thickness burns, respectively.ConclusionsWe demonstrated that partial- and full-thickness burn wounds can be precisely and reproducibly created with our new automated burning device.

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