• Sepideh Esfahani Moghaddam, Ali Barzegar, and Novin Nikbakhsh.
• Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
• J Res Med Sci. 2017 Jan 1; 22: 21.

BackgroundThe incidence rate of breast cancer has been dramatically increasing since the last decade in Iran, and it is now one of the most common female malignant tumors. B-cell lymphoma 2 (BCL2) family is the most important regulator of apoptosis, and -938C>A single nucleotide polymorphism (SNP) of BCL2 gene promoter has been demonstrated to influence breast cancer susceptibility. In this research, we study the effect of -938C>A allelic variants on breast cancer risk in Mazandaran province at the North of Iran.Materials And MethodsThis analysis performed on 120 breast cancer patients who underwent surgery in some referenced hospitals at Mazandaran province along with 130 healthy individuals as a control. DNA extracted from peripheral blood samples was applied in polymerase chain reaction-single-strand conformation polymorphism analysis to determine -938C>A genotype. The association of the -938C>A genotype and breast cancer risk as well as clinicopathological characters were analyzed by logistic regression method.ResultsResults showed that genotype frequency of AA, AC, and CC genotypes was 10%, 62%, and 28% for case and 28%, 50%, and 22% in control group, respectively. In the logistic regression model, BCL2 - 938C/A variant genotype AA was associated with a decreased risk of breast cancer (P = 0.041) by 0.31-fold (odds ratio = 0.31, confidence interval = 0.091-0.909) compared to CC genotype. However, no significant association found between -938C>A genotype and clinicopathological characters.ConclusionThe study showed that AA genotype of BCL2 gene (-938C>A) is associated with decreased susceptibility to breast cancer. Hence, investigating the -938C>A SNP of BCL2 gene promoter could be an appropriate molecular marker to determine individual sensitivity to breast cancer.

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