• Kuo-Chou Chiu, Yin-Hwa Shih, Tong-Hong Wang, Wan-Chen Lan, Po-Jung Li, Hong-Syu Jhuang, Shih-Min Hsia, Yen-Wen Shen, Michael Yuan-Chien Chen, and Tzong-Ming Shieh.
• Division of Oral Diagnosis and Family Dentistry, National Defense Medical Center, Taipei, Taiwan.
• J Formos Med Assoc. 2021 Feb 1; 120 (2): 827-837.

Background/PurposeHonokiol and magnolol are natural components isolated from Magnolia bark that is used in traditional Chinese and Japanese herbal medicine. These two isomers are used as a component of dietary supplements and cosmetic products. In this study, we investigated the antimicrobial effect of honokiol and magnolol on pathogens causing oral diseases, their mechanism of action in biofilm formation and drug resistance of oral pathogens, and inflammatory regulation in mammalian cells.MethodsWe determined the minimum inhibitory concentration and minimum bactericidal concentration of honokiol and magnolol, and their stability at different temperatures and pH. We also evaluated their effect on biofilm formation, antibiotic-resistance gene expression in MRSA, and pro-inflammatory gene expression in mammalian cells.ResultsHonokiol showed better antimicrobial activity than magnolol. Both honokiol and magnolol showed stable bacterial inhibitory activity over a wide range of temperature and pH, reduced biofilm formation, and antibiotic resistance in oral pathogens. The biofilm formation- and antibiotic resistance-related gene expression was consistent with the respective phenotypes. Furthermore, these two isomers repressed the expression of pro-inflammatory genes in RAW264.7 cells.ConclusionOur study provides evidence of the potential application of honokiol and magnolol in dental medicine to cure or prevent oral diseases.Copyright © 2020 Formosan Medical Association. Published by Elsevier B.V. All rights reserved.

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