• Maximilian Leiblein, Andreas Winkenbach, Elias Koch, Alexander Schaible, Hubert Büchner, Ingo Marzi, Dirk Henrich, and Christoph Nau.
• Klinik für Unfall-, Hand- und Wiederherstellungschirurgie, Klinikum der Johann Wolfgang Goethe-Universität, Frankfurt am Main, Germany. maximilian.leiblein@kgu.de.
• Eur J Trauma Emerg Surg. 2022 Feb 1; 48 (1): 679-687.

PurposeThe Masquelet technique for the treatment of large bone defects is a two-stage procedure based on an induced membrane. Compared to mature periosteum, the induced membrane differs significantly. However, both play a crucial role in bone regeneration. As part of a histological and radiological post-evaluation of an earlier project, we analyzed the influence of the granule size of the bone void filler Herafill® on development of periosteum regrowth in a critical size defect.MethodsWe compared three different sizes of Herafill® granules (Heraeus Medical GmbH, Wehrheim) in vivo in a rat femoral critical size defect (10 mm) treated with the induced membrane technique. After 8 weeks healing time, femurs were harvested and taken for histological and radiological analysis.ResultsA significantly increased regrowth of periosteum into the defect was found when small granules were used. Large granules showed significantly increased occurrence of bone capping. Small granules lead to significant increase in callus formation in the vicinity to the membrane.ConclusionThe size of Herafill® granules has significant impact on the development of periosteal-like structures around the defect using Masquelet's induced membrane technique. Small granules show significantly increased regrowth of periosteum and improved bone formation adjacent to the induced membrane.© 2020. The Author(s).

7 keywords...

hide…