• Joseph A Roche, Morium Begam, Andrea K Eaton, Collin J Elkins, Jaclyn P Johnson, Mattina M Rosinski, and Sujay S Galen.
• Physical Therapy Program, Department of Health Care Sciences, Wayne State University, 259 Mack Ave. Rm 4440. Detroit, MI 48201.
• Mil Med. 2020 Jan 7; 185 (Suppl 1): 423-429.

IntroductionThe aim of this study was to quantify the extent of donor-cell-derived myogenesis achieved by a novel surgical technique known as Minimally Invasive Muscle Embedding (MIME).Materials And MethodsThrough MIME, we implanted a single extensor digitorum longus muscle from donor mice (N = 2) that expressed a red fluorescent protein (RFP), into the left tibialis anterior (TA) muscle of immunodeficient host mice (N = 4) that expressed a green fluorescent protein (GFP). Soon after MIME, we injected a myotoxin (barium chloride), into the host TA muscle, to trigger concerted muscle degeneration and regeneration. In lieu of MIME, we performed a SHAM procedure on the right TA muscle of the same set of animals.ResultsIn MIME-treated muscles, 22% ± 7% and 78% ± 7% muscle fibers were RFP+ and GFP+, respectively (mean ± standard deviation); and all RFP+ fibers were positive for desmin and dystrophin. Conclusion. We conclude that MIME helps generate muscle fibers of donor origin, in host muscle.Published by Oxford University Press on behalf of Association of Military Surgeons of the United States 2020.

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