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J. Clin. Microbiol. · Sep 1998
Early detection of Mycobacterium tuberculosis in BACTEC cultures by ligase chain reaction.
- E Tortoli, F Lavinia, and M T Simonetti.
- Laboratorio di Microbiologia e Virologia, Ospedale di Careggi, 50139 Florence, Italy. tortoli@dada.it
- J. Clin. Microbiol. 1998 Sep 1; 36 (9): 2791-2.
AbstractThe LCx Mycobacterium tuberculosis ligase chain reaction system (Abbott Diagnostic Division, Abbott Park, Ill.) was used to detect M. tuberculosis in 150 consecutive BACTEC vials on the day on which a positive growth index (GI) was recorded. By LCx, M. tuberculosis DNA was detected in BACTEC vials on average 2.6 days before the presence of acid-fast bacilli could be confirmed by microscopic examination. A total of 106 of 108 M. tuberculosis isolates were detected without centrifugation from bottles presenting very low GIs (average, 70; median, 33). No false-positive result was obtained from nontuberculous mycobacteria or from isolates with contaminants.
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