• Ömer Bayır, Tuğba Karagöz, Ferda Alpaslan Pınarlı, Gülistan Sanem Sarıbaş, Candan Özoğul, Kemal Keseroğlu, Güleser Saylam, Emel Çadallı Tatar, Sevilay Karahan, Bülent Öcal, and Mehmet Hakan Korkmaz.
• Department of Otolaryngology Head and Neck Surgery, University of Health Sciences, Dışkapı Yıldırım Beyazıt Training and Research Hospital, Ankara, Turkey
• Turk J Med Sci. 2021 Jun 28; 51 (3): 1481-1490.

Background/AimTo evaluate the clinical and histopathological effects of fetal brain tissue derived mesenchymal stem cells (FBTMSC) and fibrin glue (FG) on the facial nerve (FN) regeneration in rats with traumatic FN injury.Materials And MethodsTwenty-eight Sprague Dawley rats were included in the study and divided into 4 groups. Traumatic FN injury (FP) was created by a surgical clamp compression to the main trunk of left FN in all groups. In the control group (group 1) no treatment was applied, in group 2 (FBTMSC group) 2 × 106 FBTMSC was injected, in group 3 (FG group) only FG was applied, in group 4 (FBTMSC and FG groups) both FBTMSC and FG were applied to the injured section of the nerve. The FN functions were evaluated clinically, immediately after the procedure and at 3rd, 5th, and 8th weeks postoperatively. The FNs of all subjects were excised after the 8th week; then the rats were sacrificed. The presence of stem cells in the injured zone was assessed using bromo-deoxyuridine (BrdU), and apoptosis was determined by the TUNEL method.ResultsAfter the damage, total FP was observed in all subjects. Statistically significant functional improvement was observed in group 4 compared to all other groups (P < 0.005). TUNEL-positive cell count was statistically significantly higher in the control group than the other groups (P < 0.001). TUNEL-positive cell count was statistically significantly lower in group 4 than the other groups. The proportion of BrdU-stained cells in group 4 (5%) was higher than group 2 (2%).ConclusionClinically and histopathologically FBTMSC applied with FG may play a promising role as a regenerative treatment in posttraumatic FP.This work is licensed under a Creative Commons Attribution 4.0 International License.

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