• J. Clin. Virol. · Sep 2015

    Performance of the Alere i influenza A&B assay and mariPOC test for the rapid detection of influenza A and B viruses.

    • Pia Jokela, Tytti Vuorinen, Matti Waris, and Raija Manninen.
    • Laboratory Services (Satadiag), Satakunta Central Hospital, 28500 Pori, Finland; Department of Clinical Virology, Division of Microbiology and Genetics, Turku University Hospital, 20521 Turku, Finland. Electronic address: pia.jokela@helsinki.fi.
    • J. Clin. Virol. 2015 Sep 1; 70: 72-76.

    BackgroundTimely detection of influenza viruses is required to facilitate infection control measures and appropriate patient management. The Alere™ i Influenza A&B assay for detection of viral RNA and multianalyte mariPOC(®) test for detection of viral antigens enable rapid detection of influenza viruses with little hands-on time.ObjectivesTo evaluate the performance of the Alere i Influenza A&B assay and the mariPOC test in comparison to the Xpert(®) Flu A/B assay and laboratory-developed real-time reverse transcription-polymerase chain reaction.Study DesignA total of 140 and 108 nasopharyngeal specimens were analysed for evaluation of the Alere i and mariPOC, respectively.ResultsThe sensitivity and specificity of the Alere i Influenza A&B assay for detection of influenza A virus was 80.0% and 98.1%, and for influenza B virus 45.2% and 98.2%, respectively. For the mariPOC test, a sensitivity and specificity of 53.1% and 98.7%, respectively, for detection of influenza A virus was achieved.ConclusionsThe mariPOC test proved insensitive for detection of influenza A virus and therefore unsuitable for individual patient diagnosis without confirmatory testing. In contrast, the Alere i Influenza A&B assay was reasonably sensitive and specific for detection of influenza A and B virus, although decreased detection of specimens with low viral load was observed particularly for detection of influenza B virus. Taken together with its rapidity and ease of use, the Alere i influenza A&B assay is a welcome alternative to immunochromatographic rapid influenza detection tests and may provide timely results that enable appropriate patient care and management of patient flow during high-prevalence seasons.Copyright © 2015 Elsevier B.V. All rights reserved.

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